Proteome analysis of coinfection of epithelial cells with Filifactor alocis and Porphyromonas gingivalis shows modulation of pathogen and host regulatory pathways

A. Wilson Aruni, Kangling Zhang, Yuetan Dou, Hansel Fletcher

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Changes in periodontal status are associated with shifts in the composition of the bacterial community in the periodontal pocket. The relative abundances of several newly recognized microbial species, including Filifactor alocis, as-yet-unculturable organisms, and other fastidious organisms have raised questions on their impact on disease development. We have previously reported that the virulence attributes of F. alocis are enhanced in coculture with Porphyromonas gingivalis. We have evaluated the proteome of host cells and F. alocis during a polymicrobial infection. Coinfection of epithelial cells with F. alocis and P. gingivalis strains showed approximately 20% to 30% more proteins than a monoinfection. Unlike F. alocis ATCC 35896, the D-62D strain expressed more proteins during coculture with P. gingivalis W83 than with P. gingivalis 33277. Proteins designated microbial surface component-recognizing adhesion matrix molecules (MSCRAMMs) and cell wall anchor proteins were highly upregulated during the polymicrobial infection. Ultrastructural analysis of the epithelial cells showed formation of membrane microdomains only during coinfection. The proteome profile of epithelial cells showed proteins related to cytoskeletal organization and gene expression and epigenetic modification to be in high abundance. Modulation of proteins involved in apoptotic and cell signaling pathways was noted during coinfection. The enhanced virulence potential of F. alocis may be related to the differential expression levels of several putative virulence factors and their effects on specific host cell pathways.

Original languageEnglish (US)
Pages (from-to)3261-3274
Number of pages14
JournalInfection and Immunity
Volume82
Issue number8
DOIs
StatePublished - 2014

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Porphyromonas gingivalis
Proteome
Coinfection
Epithelial Cells
Proteins
Coculture Techniques
Virulence
Membrane Microdomains
Periodontal Pocket
Virulence Factors
Epigenomics
Cell Wall
Gene Expression

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Parasitology
  • Infectious Diseases

Cite this

Proteome analysis of coinfection of epithelial cells with Filifactor alocis and Porphyromonas gingivalis shows modulation of pathogen and host regulatory pathways. / Aruni, A. Wilson; Zhang, Kangling; Dou, Yuetan; Fletcher, Hansel.

In: Infection and Immunity, Vol. 82, No. 8, 2014, p. 3261-3274.

Research output: Contribution to journalArticle

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