Proteomic analysis of Pichindé virus infection identifies differential expression of prothymosin-alpha.

Gavin C. Bowick, Kizhake V. Soman, H. Wang, Judith F. Aronson, Bruce A. Luxon, Lee O. Lomas, David G. Gorenstein, Norbert K. Herzog

Research output: Contribution to journalArticle

Abstract

The arenaviruses include a number of important pathogens including Lassa virus and Junin virus. Presently, the only treatment is supportive care and the antiviral Ribavirin. In the event of an epidemic, patient triage may be required to more effectively manage resources; the development of prognostic biomarker signatures, correlating with disease severity, would allow rational triage. Using a pair of arenaviruses, which cause mild or severe disease, we analyzed extracts from infected cells using SELDI mass spectrometry to characterize potential biomarker profiles. EDGE analysis was used to analyze longitudinal expression differences. Extracts from infected guinea pigs revealed protein peaks which could discriminate between mild or severe infection, and between times post-infection. Tandem mass-spectrometry identified several peaks, including the transcriptional regulator prothymosin-alpha. Further investigation revealed differences in secretion of this peptide. These data show proof of concept that proteomic profiling of host markers could be used as prognostic markers of infectious disease.

Original languageEnglish (US)
JournalJournal of biomedicine & biotechnology
Volume2010
StatePublished - 2010
Externally publishedYes

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Arenavirus
Triage
Virus Diseases
Viruses
Proteomics
Lassa virus
Junin virus
Biomarkers
Mass spectrometry
Ribavirin
Tandem Mass Spectrometry
Infection
Cell Extracts
Antiviral Agents
Communicable Diseases
Mass Spectrometry
Guinea Pigs
Pathogens
Peptides
Proteins

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Proteomic analysis of Pichindé virus infection identifies differential expression of prothymosin-alpha. / Bowick, Gavin C.; Soman, Kizhake V.; Wang, H.; Aronson, Judith F.; Luxon, Bruce A.; Lomas, Lee O.; Gorenstein, David G.; Herzog, Norbert K.

In: Journal of biomedicine & biotechnology, Vol. 2010, 2010.

Research output: Contribution to journalArticle

Bowick, GC, Soman, KV, Wang, H, Aronson, JF, Luxon, BA, Lomas, LO, Gorenstein, DG & Herzog, NK 2010, 'Proteomic analysis of Pichindé virus infection identifies differential expression of prothymosin-alpha.', Journal of biomedicine & biotechnology, vol. 2010.
Bowick, Gavin C. ; Soman, Kizhake V. ; Wang, H. ; Aronson, Judith F. ; Luxon, Bruce A. ; Lomas, Lee O. ; Gorenstein, David G. ; Herzog, Norbert K. / Proteomic analysis of Pichindé virus infection identifies differential expression of prothymosin-alpha. In: Journal of biomedicine & biotechnology. 2010 ; Vol. 2010.
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AU - Luxon, Bruce A.

AU - Lomas, Lee O.

AU - Gorenstein, David G.

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