Proteomic analysis of the asthmatic airway

John E. Wiktorowicz, Mohammad Jamaluddin

Research output: Chapter in Book/Report/Conference proceedingChapter

16 Scopus citations


Proteomic investigations in general utilize varied technologies for sample preparation, separations, quantification, protein identification, and biological rationalization. Their applications range from pure discovery and mechanistic studies to biomarker discovery/verification/validation. In each specific case, the analytical strategy to be implemented is tailored to the type of sample that serves as the target of the investigations. Proteomic investigations take into consideration sample complexity, the cellular heterogeneity (particularly from tissues), the potential dynamic range of the protein and peptide abundance within the sample, the likelihood of posttranslational modifications (PTM), and other important factors that might influence the final output of the study. We describe the sample types typically used for proteomic investigations into the biology of asthma and review the most recent related publications with special attention to those that deal with the unique airway samples such as bronchoalveolar lavage fluids (BALF), epithelial lining fluid and cells (ELF), induced sputum (IS), and exhaled breath condensate (EBC). Finally, we describe the newest proteomics approaches to sample preparation of the unique airway samples, BALF and IS.

Original languageEnglish (US)
Title of host publicationHeterogeneity in Asthma
PublisherSpringer New York LLC
Number of pages12
ISBN (Print)9781461486022
StatePublished - 2014

Publication series

NameAdvances in Experimental Medicine and Biology
ISSN (Print)0065-2598


  • Airway inflammation
  • Asthma
  • Bronchoalveolar lavage
  • Bronchoscopic microsampling
  • Epithelial lining fluid
  • Exhaled breath condensate
  • Induced sputum
  • Proteomics
  • Sample prep
  • Size-exclusion chromatography

ASJC Scopus subject areas

  • General Biochemistry, Genetics and Molecular Biology


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