Abstract
Proteomic investigations in general utilize varied technologies for sample preparation, separations, quantification, protein identification, and biological rationalization. Their applications range from pure discovery and mechanistic studies to biomarker discovery/verification/validation. In each specific case, the analytical strategy to be implemented is tailored to the type of sample that serves as the target of the investigations. Proteomic investigations take into consideration sample complexity, the cellular heterogeneity (particularly from tissues), the potential dynamic range of the protein and peptide abundance within the sample, the likelihood of posttranslational modifications (PTM), and other important factors that might influence the final output of the study. We describe the sample types typically used for proteomic investigations into the biology of asthma and review the most recent related publications with special attention to those that deal with the unique airway samples such as bronchoalveolar lavage fluids (BALF), epithelial lining fluid and cells (ELF), induced sputum (IS), and exhaled breath condensate (EBC). Finally, we describe the newest proteomics approaches to sample preparation of the unique airway samples, BALF and IS.
| Original language | English (US) |
|---|---|
| Title of host publication | Advances in Experimental Medicine and Biology |
| Publisher | Springer New York LLC |
| Pages | 221-232 |
| Number of pages | 12 |
| Volume | 795 |
| ISBN (Print) | 9781461486022 |
| DOIs | |
| State | Published - 2014 |
Publication series
| Name | Advances in Experimental Medicine and Biology |
|---|---|
| Volume | 795 |
| ISSN (Print) | 00652598 |
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Keywords
- Airway inflammation
- Asthma
- Bronchoalveolar lavage
- Bronchoscopic microsampling
- Epithelial lining fluid
- Exhaled breath condensate
- Induced sputum
- Proteomics
- Sample prep
- Size-exclusion chromatography
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Medicine(all)
Cite this
Proteomic analysis of the asthmatic airway. / Wiktorowicz, John E.; Jamaluddin, Mohammad.
Advances in Experimental Medicine and Biology. Vol. 795 Springer New York LLC, 2014. p. 221-232 (Advances in Experimental Medicine and Biology; Vol. 795).Research output: Chapter in Book/Report/Conference proceeding › Chapter
}
TY - CHAP
T1 - Proteomic analysis of the asthmatic airway
AU - Wiktorowicz, John E.
AU - Jamaluddin, Mohammad
PY - 2014
Y1 - 2014
N2 - Proteomic investigations in general utilize varied technologies for sample preparation, separations, quantification, protein identification, and biological rationalization. Their applications range from pure discovery and mechanistic studies to biomarker discovery/verification/validation. In each specific case, the analytical strategy to be implemented is tailored to the type of sample that serves as the target of the investigations. Proteomic investigations take into consideration sample complexity, the cellular heterogeneity (particularly from tissues), the potential dynamic range of the protein and peptide abundance within the sample, the likelihood of posttranslational modifications (PTM), and other important factors that might influence the final output of the study. We describe the sample types typically used for proteomic investigations into the biology of asthma and review the most recent related publications with special attention to those that deal with the unique airway samples such as bronchoalveolar lavage fluids (BALF), epithelial lining fluid and cells (ELF), induced sputum (IS), and exhaled breath condensate (EBC). Finally, we describe the newest proteomics approaches to sample preparation of the unique airway samples, BALF and IS.
AB - Proteomic investigations in general utilize varied technologies for sample preparation, separations, quantification, protein identification, and biological rationalization. Their applications range from pure discovery and mechanistic studies to biomarker discovery/verification/validation. In each specific case, the analytical strategy to be implemented is tailored to the type of sample that serves as the target of the investigations. Proteomic investigations take into consideration sample complexity, the cellular heterogeneity (particularly from tissues), the potential dynamic range of the protein and peptide abundance within the sample, the likelihood of posttranslational modifications (PTM), and other important factors that might influence the final output of the study. We describe the sample types typically used for proteomic investigations into the biology of asthma and review the most recent related publications with special attention to those that deal with the unique airway samples such as bronchoalveolar lavage fluids (BALF), epithelial lining fluid and cells (ELF), induced sputum (IS), and exhaled breath condensate (EBC). Finally, we describe the newest proteomics approaches to sample preparation of the unique airway samples, BALF and IS.
KW - Airway inflammation
KW - Asthma
KW - Bronchoalveolar lavage
KW - Bronchoscopic microsampling
KW - Epithelial lining fluid
KW - Exhaled breath condensate
KW - Induced sputum
KW - Proteomics
KW - Sample prep
KW - Size-exclusion chromatography
UR - http://www.scopus.com/inward/record.url?scp=84934441007&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84934441007&partnerID=8YFLogxK
U2 - 10.1007/978-1-4614-8603-9_14
DO - 10.1007/978-1-4614-8603-9_14
M3 - Chapter
SN - 9781461486022
VL - 795
T3 - Advances in Experimental Medicine and Biology
SP - 221
EP - 232
BT - Advances in Experimental Medicine and Biology
PB - Springer New York LLC
ER -