Proteomic analysis of the asthmatic airway

John E. Wiktorowicz, Mohammad Jamaluddin

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Proteomic investigations in general utilize varied technologies for sample preparation, separations, quantification, protein identification, and biological rationalization. Their applications range from pure discovery and mechanistic studies to biomarker discovery/verification/validation. In each specific case, the analytical strategy to be implemented is tailored to the type of sample that serves as the target of the investigations. Proteomic investigations take into consideration sample complexity, the cellular heterogeneity (particularly from tissues), the potential dynamic range of the protein and peptide abundance within the sample, the likelihood of posttranslational modifications (PTM), and other important factors that might influence the final output of the study. We describe the sample types typically used for proteomic investigations into the biology of asthma and review the most recent related publications with special attention to those that deal with the unique airway samples such as bronchoalveolar lavage fluids (BALF), epithelial lining fluid and cells (ELF), induced sputum (IS), and exhaled breath condensate (EBC). Finally, we describe the newest proteomics approaches to sample preparation of the unique airway samples, BALF and IS.

Original languageEnglish (US)
Title of host publicationAdvances in Experimental Medicine and Biology
Pages221-232
Number of pages12
Volume795
DOIs
StatePublished - 2014

Publication series

NameAdvances in Experimental Medicine and Biology
Volume795
ISSN (Print)00652598

Keywords

  • Airway inflammation
  • Asthma
  • Bronchoalveolar lavage
  • Bronchoscopic microsampling
  • Epithelial lining fluid
  • Exhaled breath condensate
  • Induced sputum
  • Proteomics
  • Sample prep
  • Size-exclusion chromatography

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

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  • Cite this

    Wiktorowicz, J. E., & Jamaluddin, M. (2014). Proteomic analysis of the asthmatic airway. In Advances in Experimental Medicine and Biology (Vol. 795, pp. 221-232). (Advances in Experimental Medicine and Biology; Vol. 795). https://doi.org/10.1007/978-1-4614-8603-9-14