Proteomic analysis to identify the role of luxS/AI-2 mediated protein expression in Escherichia coli O157

H7

Kamlesh Soni, Palmy Jesudhasan, Martha Cepeda, Brad Williams, Michael Hume, William Russell, Arul Jayaraman, Suresh D. Pillai

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Microorganisms employ autoinducer molecules to modulate various bacterial processes including virulence expression, biofilm development, and bioluminescence. The universal autoinducer molecule AI-2 is hypothesized to mediate cell signaling in Escherichia coli O157:H7. We investigated the role of AI-2 on the E. coli O157:H7 cellular proteins using a two-dimensional (2D) gel electrophoresis-based proteomic approach. The protein expression patterns between two experimental comparisons were studied namely, 1) a wild type E. coli O157:H7 and its isogenic luxS mutant, and 2) the luxS mutant and the luxS mutant supplemented with AI-2 molecules. Eleven proteins were differentially expressed between the wild type and the luxS mutant strain, whereas 18 proteins were differentially expressed in the luxS mutant strain when supplemented with AI-2. The tryptophan repressor binding protein (WrbA), phosphoglycerate mutase (GpmA), and a putative protein YbbN were found to be differentially expressed under both experimental comparisons. The FliC protein which is involved in flagellar synthesis and motility was up-regulated in the wild type strain but was not influenced by the addition of synthetic AI-2 molecules to the luxS mutant suggesting the involvement of signaling molecules other than AI-2 on flagellar synthesis and motility.

Original languageEnglish (US)
Pages (from-to)463-471
Number of pages9
JournalFoodborne Pathogens and Disease
Volume4
Issue number4
DOIs
StatePublished - Nov 1 2007
Externally publishedYes

Fingerprint

Escherichia coli O157
Proteomics
proteomics
protein synthesis
mutants
Proteins
Electrophoresis, Gel, Two-Dimensional
proteins
Bacterial Physiological Phenomena
Phosphoglycerate Mutase
phosphoglyceric acids
synthesis
bioluminescence
two-dimensional gel electrophoresis
Biofilms
tryptophan
biofilm
Virulence
binding proteins
Carrier Proteins

ASJC Scopus subject areas

  • Food Science
  • Applied Microbiology and Biotechnology
  • Microbiology
  • Infectious Diseases

Cite this

Proteomic analysis to identify the role of luxS/AI-2 mediated protein expression in Escherichia coli O157 : H7. / Soni, Kamlesh; Jesudhasan, Palmy; Cepeda, Martha; Williams, Brad; Hume, Michael; Russell, William; Jayaraman, Arul; Pillai, Suresh D.

In: Foodborne Pathogens and Disease, Vol. 4, No. 4, 01.11.2007, p. 463-471.

Research output: Contribution to journalArticle

Soni, Kamlesh ; Jesudhasan, Palmy ; Cepeda, Martha ; Williams, Brad ; Hume, Michael ; Russell, William ; Jayaraman, Arul ; Pillai, Suresh D. / Proteomic analysis to identify the role of luxS/AI-2 mediated protein expression in Escherichia coli O157 : H7. In: Foodborne Pathogens and Disease. 2007 ; Vol. 4, No. 4. pp. 463-471.
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