Proteomic studies of potential cerebrospinal fluid protein markers for Alzheimer's disease

Maja Puchades, Sara Folkesson Hansson, Carol L. Nilsson, Niels Andreasen, Kaj Blennow, Pia Davidsson

Research output: Contribution to journalArticle

250 Scopus citations

Abstract

By comparing the cerebrospinal fluid (CSF) proteome between Alzheimer's disease (AD) patients and controls, it may be possible to identify proteins that play a role in the disease process and thus to study the pathogenesis of AD. Two-dimensional gel electrophoresis (2-DE), SYPRO Ruby staining and mass spectrometry were used for clinical screening of disease-influenced CSF proteins in AD patients compared to controls. In order to increase the detection of CSF proteins and to improve the separation of protein isoforms in a 2-D gel, micro-narrow range IPG strips were used. The levels of eight proteins and their isoforms, including apolipoprotein A1, apolipoprotein E, apolipoprotein J, β-trace, retinol-binding protein, kininogen, α-1 antitrypsin, cell cycle progression 8 protein, and α-1β glycoprotein were significantly altered in CSF of AD patients compared to controls. Furthermore, we also used liquid-phase IEF, as a prefractionation step, prior to 2-DE for comparison of CSF proteins between individual AD patients and controls. The levels of 37 proteins spots were altered in AD patients. One of the identified proteins, α-2-HS glycoprotein, has not previously been linked to AD. Our study shows that several glycoproteins are altered in AD.

Original languageEnglish (US)
Pages (from-to)140-146
Number of pages7
JournalMolecular Brain Research
Volume118
Issue number1-2
DOIs
StatePublished - Oct 21 2003

Keywords

  • 2-DE
  • Alzheimer's disease
  • Cerebrospinal fluid
  • Liquid-phase IEF
  • Mass spectrometry
  • Proteomics

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience

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  • Cite this

    Puchades, M., Hansson, S. F., Nilsson, C. L., Andreasen, N., Blennow, K., & Davidsson, P. (2003). Proteomic studies of potential cerebrospinal fluid protein markers for Alzheimer's disease. Molecular Brain Research, 118(1-2), 140-146. https://doi.org/10.1016/j.molbrainres.2003.08.005