Proteomic Techniques for Functional Identification of Bacterial Adhesins

Elisabet Carlsohn, Carol L. Nilsson

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)

Abstract

This chapter presents an overview of techniques used in proteomics and describes a functional proteomic approach for identification of a lactoferrin-binding protein of Helicobacter pylori. Proteomics is usually divided into expression proteomics, which includes analysis of protein expression, as well as quantification, functional and structural characterization of proteins in cell lysates and tissues, and cell-map proteomics, which attempts to define all protein-protein interactions and intracellular signaling that occurs in a cell under a given condition. Expression proteomics relies heavily on two-dimensional gel electrophoresis (2D-GE) computer analysis to reveal patterns of protein expression and mass spectrometric analysis of enzymatically cleaved peptides. Cell-mapping proteomics is performed either by affinity purification and identification of protein complexes by mass spectrometry (MS) or by direct DNA readout by yeast two-hybrid, phage display, ribosome display, and RNA-peptide fusion.

Original languageEnglish (US)
Title of host publicationLectins: Analytical Technologies
PublisherElsevier
Pages299-325
Number of pages27
ISBN (Print)9780444530776
DOIs
StatePublished - 2007
Externally publishedYes

Fingerprint

Bacterial Adhesins
Proteins
Display devices
Peptides
Bacteriophages
Lactoferrin
Proteomics
Electrophoresis
Yeast
Purification
Mass spectrometry
Carrier Proteins
Fusion reactions
Gels
RNA
Tissue
DNA

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Carlsohn, E., & Nilsson, C. L. (2007). Proteomic Techniques for Functional Identification of Bacterial Adhesins. In Lectins: Analytical Technologies (pp. 299-325). Elsevier. https://doi.org/10.1016/B978-044453077-6/50013-2

Proteomic Techniques for Functional Identification of Bacterial Adhesins. / Carlsohn, Elisabet; Nilsson, Carol L.

Lectins: Analytical Technologies. Elsevier, 2007. p. 299-325.

Research output: Chapter in Book/Report/Conference proceedingChapter

Carlsohn, E & Nilsson, CL 2007, Proteomic Techniques for Functional Identification of Bacterial Adhesins. in Lectins: Analytical Technologies. Elsevier, pp. 299-325. https://doi.org/10.1016/B978-044453077-6/50013-2
Carlsohn E, Nilsson CL. Proteomic Techniques for Functional Identification of Bacterial Adhesins. In Lectins: Analytical Technologies. Elsevier. 2007. p. 299-325 https://doi.org/10.1016/B978-044453077-6/50013-2
Carlsohn, Elisabet ; Nilsson, Carol L. / Proteomic Techniques for Functional Identification of Bacterial Adhesins. Lectins: Analytical Technologies. Elsevier, 2007. pp. 299-325
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