@inbook{1aaa294d418346c7b5b42889cda9f88b,
title = "Protocols to Measure Oxidative Stress and DNA Damage in Asthma",
abstract = "Asthma is associated with oxidative stress and oxidative damage of biomolecules, including DNA. Here, we describe the protocols to quantify reactive oxygen species (ROS) and oxidative stress markers in a mouse model of allergic airway inflammation. We also provide detailed methods to measure DNA damage by long-run real-time PCR for DNA-damage quantification (LORD-Q) assay and gene-specific DNA damage analyses by long amplicon (LA)-qPCR. Additionally, we describe methods to quantify oxidized DNA base lesions in lung genomic DNA by mass spectrometry, and to measure enzymatic activity of 8-oxoguanine DNA glycosylase (OGG1). Using these methods, the levels of oxidative stress and DNA damage in allergic inflammation and asthma can be elucidated.",
keywords = "Allergic inflammation, Asthma, GSSG, LA-PCR, LORD-Q PCR, NEIL2, OGG1, Oxidative DNA damage, Reactive oxygen species",
author = "Koa Hosoki and Anirban Chakraborty and Hazra, {Tapas K.} and Sanjiv Sur",
note = "Funding Information: This research was supported in parts by the National Heart, Lung, and Blood Institute (grant nos. 5R01HL145477-02 and 3R01HL145477-01S1) and the Department of Defense (grant no. PR171425 W81XWH-18-1-0743). Funding Information: We thank Dr. Pawel Jaruga, Dr. Erdem Coskun, and Dr. Miral Dizdaroglu (Biomolecular Measurement Division National Institute of Standards and Technology, Gaithersburg, Maryland, USA) for their scientific input and for critical writing and editing of the manuscript. We also thank Dr. Nisha Tapryal (Department of Internal Medicine, Division of Pulmonary, Critical Care and Sleep Medicine, University of Texas Medical Branch, Galveston, Texas, USA) for her scientific input and for critical writing and editing of the manuscript. This research was supported in parts by the National Heart, Lung, and Blood Institute (grant nos. 5R01HL145477-02 and 3R01HL145477-01S1) and the Department of Defense (grant no. PR171425 W81XWH-18-1-0743). Publisher Copyright: {\textcopyright} 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2022",
doi = "10.1007/978-1-0716-2364-0_22",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "315--332",
booktitle = "Methods in Molecular Biology",
}