@inbook{1aaa294d418346c7b5b42889cda9f88b,
title = "Protocols to Measure Oxidative Stress and DNA Damage in Asthma",
abstract = "Asthma is associated with oxidative stress and oxidative damage of biomolecules, including DNA. Here, we describe the protocols to quantify reactive oxygen species (ROS) and oxidative stress markers in a mouse model of allergic airway inflammation. We also provide detailed methods to measure DNA damage by long-run real-time PCR for DNA-damage quantification (LORD-Q) assay and gene-specific DNA damage analyses by long amplicon (LA)-qPCR. Additionally, we describe methods to quantify oxidized DNA base lesions in lung genomic DNA by mass spectrometry, and to measure enzymatic activity of 8-oxoguanine DNA glycosylase (OGG1). Using these methods, the levels of oxidative stress and DNA damage in allergic inflammation and asthma can be elucidated.",
keywords = "Allergic inflammation, Asthma, GSSG, LA-PCR, LORD-Q PCR, NEIL2, OGG1, Oxidative DNA damage, Reactive oxygen species",
author = "Koa Hosoki and Anirban Chakraborty and Hazra, {Tapas K.} and Sanjiv Sur",
note = "Publisher Copyright: {\textcopyright} 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2022",
month = jul,
day = "1",
doi = "10.1007/978-1-0716-2364-0_22",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "315--332",
booktitle = "Methods in Molecular Biology",
}