Proton pumps, angiogenesis, and metastatic breast cancer

Jose Rojas, Shankar C. Sanka, Defeng Luo, Christian Busch, Gloria M. Martinez, Mary J C Hendrix, Raul Martinez-Zaguilan

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Citations (Scopus)

Abstract

We have previously shown the relationship between metastatic potential and plasmalemmal V-H +-ATPase (pm V-ATPase) expression in tumor cells. This led us to hypothesize that pm V-ATPase activity is involved in invasion. Angiogenesis involves invasion of adjacent tissues by microvascular endothelial cells, thus we hypothesized that pm V-ATPases contribute to pH in regulation and invasion in microvascular endothelial cells. Immunocytochemical experiments revealed pm V-ATPases at the leading edge of cancer and microvascular endothelial cells. We monitored pH in from leading- to lagging-edge at the single cell level using confocal/spectral imaging microscopy. Our data indicate that the pH in is more alkaline at the leading than at the lagging edge, in both these cell types, suggesting the presence of pH in gradients. Line scanning confocal microscopy demonstrated pH in oscillations whose magnitude were larger in the lagging than the leading edge. Exposure of the cell's leading edge to bafilomycin A 1, a V-H +-ATPase inhibitor, decreased the migratory capabilities of the cells. Inhibitors for P- and F-type ATPases had no effect on these phenomena. Altogether, these data indicate that pm V-ATPases play a role in pH in regulation and invasion in tumor and microvascular endothelial cells. This could offer a potential target to modulate angiogenesis and metastasis.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
PublisherSPIE
Pages67-78
Number of pages12
Volume3924
StatePublished - 2000
Externally publishedYes
EventMolecular Imaging: Reporters, Dyes, Markers, and Instrumentation - San Jose, CA, USA
Duration: Jan 23 2000Jan 24 2000

Other

OtherMolecular Imaging: Reporters, Dyes, Markers, and Instrumentation
CitySan Jose, CA, USA
Period1/23/001/24/00

Fingerprint

angiogenesis
breast
Protons
cancer
Pumps
pumps
Endothelial cells
protons
leading edges
cells
inhibitors
tumors
Tumors
microscopy
metastasis
Confocal microscopy
Adenosine Triphosphatases
Microscopic examination
Cells
gradients

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

Cite this

Rojas, J., Sanka, S. C., Luo, D., Busch, C., Martinez, G. M., Hendrix, M. J. C., & Martinez-Zaguilan, R. (2000). Proton pumps, angiogenesis, and metastatic breast cancer. In Proceedings of SPIE - The International Society for Optical Engineering (Vol. 3924, pp. 67-78). SPIE.

Proton pumps, angiogenesis, and metastatic breast cancer. / Rojas, Jose; Sanka, Shankar C.; Luo, Defeng; Busch, Christian; Martinez, Gloria M.; Hendrix, Mary J C; Martinez-Zaguilan, Raul.

Proceedings of SPIE - The International Society for Optical Engineering. Vol. 3924 SPIE, 2000. p. 67-78.

Research output: Chapter in Book/Report/Conference proceedingChapter

Rojas, J, Sanka, SC, Luo, D, Busch, C, Martinez, GM, Hendrix, MJC & Martinez-Zaguilan, R 2000, Proton pumps, angiogenesis, and metastatic breast cancer. in Proceedings of SPIE - The International Society for Optical Engineering. vol. 3924, SPIE, pp. 67-78, Molecular Imaging: Reporters, Dyes, Markers, and Instrumentation, San Jose, CA, USA, 1/23/00.
Rojas J, Sanka SC, Luo D, Busch C, Martinez GM, Hendrix MJC et al. Proton pumps, angiogenesis, and metastatic breast cancer. In Proceedings of SPIE - The International Society for Optical Engineering. Vol. 3924. SPIE. 2000. p. 67-78
Rojas, Jose ; Sanka, Shankar C. ; Luo, Defeng ; Busch, Christian ; Martinez, Gloria M. ; Hendrix, Mary J C ; Martinez-Zaguilan, Raul. / Proton pumps, angiogenesis, and metastatic breast cancer. Proceedings of SPIE - The International Society for Optical Engineering. Vol. 3924 SPIE, 2000. pp. 67-78
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