Pseudoginsenoside-F11 attenuates morphine-induced signalling in Chinese hamster ovary-μ cells

Zhu Li, Nan Jie Xu, Chun Fu Wu, Ying Xiong, Hua Ping Fan, Wen Buo Zhang, Yue Sun, Gang Pei

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

Pseudoginsenoside-F11 (PF11), an ocotillol type saponin isolated from Panax quinquefolium L., has been shown to antagonize the behavioral actions of morphine. Biochemical experiments revealed that PF11 could inhibit diprenorphine (DIP) binding with an IC50 of ∼6.1 μM and reduced the binding potency of morphine in Chinese hamster ovary (CHO)-μ cells. Furthermore, PF11 significantly attenuated morphine-stimulated [35S]GTPγS binding in a dose dependent manner, and strongly decreased the efficacy of morphine to inhibit intracellular cAMP production. In addition, PF11 pretreatment could also significantly inhibit naloxone induced cAMP overshoot in the morphine-pretreated cells. However, PF11 per se had no effect on either [35S]GTPγS binding or intracellular cAMP accumulation. These data suggested that PF11 antagonized the morphine stimulated opioid receptor signalling directly at the cellular level.

Original languageEnglish (US)
Pages (from-to)1453-1456
Number of pages4
JournalNeuroReport
Volume12
Issue number7
DOIs
StatePublished - May 25 2001
Externally publishedYes

Keywords

  • Chinese hamster ovary (CHO)-μ cells
  • Morphine
  • Opioid receptor
  • Pseudoginsenoside-F

ASJC Scopus subject areas

  • Neuroscience(all)

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