Ptf1a, a bHLH transcriptional gene, defines GABAergic neuronal fates in cerebellum

Mikio Hoshino, Shoko Nakamura, Kiyoshi Mori, Takeshi Kawauchi, Mami Terao, Yoshiaki V. Nishimura, Akihisa Fukuda, Toshimitsu Fuse, Naoki Matsuo, Masaki Sone, Masahiko Watanabe, Haruhiko Bito, Toshio Terashima, Christopher V E Wright, Yoshiya Kawaguchi, Kazuwa Nakao, Yo Ichi Nabeshima

Research output: Contribution to journalArticle

317 Citations (Scopus)

Abstract

The molecular machinery governing glutamatergic-GABAergic neuronal subtype specification is unclear. Here we describe a cerebellar mutant, cerebelless, which lacks the entire cerebellar cortex in adults. The primary defect of the mutant brains was a specific inhibition of GABAergic neuron production from the cerebellar ventricular zone (VZ), resulting in secondary and complete loss of external germinal layer, pontine, and olivary nuclei during development. We identified the responsible gene, Ptf1a, whose expression was lost in the cerebellar VZ but was maintained in the pancreas in cerebelless. Lineage tracing revealed that two types of neural precursors exist in the cerebellar VZ: Ptf1a-expressing and -nonexpressing precursors, which generate GABAergic and glutamatergic neurons, respectively. Introduction of Ptf1a into glutamatergic neuron precursors in the dorsal telencephalon generated GABAergic neurons with representative morphological and migratory features. Our results suggest that Ptf1a is involved in driving neural precursors to differentiate into GABAergic neurons in the cerebellum.

Original languageEnglish (US)
Pages (from-to)201-213
Number of pages13
JournalNeuron
Volume47
Issue number2
DOIs
StatePublished - Jul 21 2005
Externally publishedYes

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GABAergic Neurons
Cerebellum
Genes
Olivary Nucleus
Telencephalon
Cerebellar Cortex
Pancreas
Neurons
Brain

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Hoshino, M., Nakamura, S., Mori, K., Kawauchi, T., Terao, M., Nishimura, Y. V., ... Nabeshima, Y. I. (2005). Ptf1a, a bHLH transcriptional gene, defines GABAergic neuronal fates in cerebellum. Neuron, 47(2), 201-213. https://doi.org/10.1016/j.neuron.2005.06.007

Ptf1a, a bHLH transcriptional gene, defines GABAergic neuronal fates in cerebellum. / Hoshino, Mikio; Nakamura, Shoko; Mori, Kiyoshi; Kawauchi, Takeshi; Terao, Mami; Nishimura, Yoshiaki V.; Fukuda, Akihisa; Fuse, Toshimitsu; Matsuo, Naoki; Sone, Masaki; Watanabe, Masahiko; Bito, Haruhiko; Terashima, Toshio; Wright, Christopher V E; Kawaguchi, Yoshiya; Nakao, Kazuwa; Nabeshima, Yo Ichi.

In: Neuron, Vol. 47, No. 2, 21.07.2005, p. 201-213.

Research output: Contribution to journalArticle

Hoshino, M, Nakamura, S, Mori, K, Kawauchi, T, Terao, M, Nishimura, YV, Fukuda, A, Fuse, T, Matsuo, N, Sone, M, Watanabe, M, Bito, H, Terashima, T, Wright, CVE, Kawaguchi, Y, Nakao, K & Nabeshima, YI 2005, 'Ptf1a, a bHLH transcriptional gene, defines GABAergic neuronal fates in cerebellum', Neuron, vol. 47, no. 2, pp. 201-213. https://doi.org/10.1016/j.neuron.2005.06.007
Hoshino M, Nakamura S, Mori K, Kawauchi T, Terao M, Nishimura YV et al. Ptf1a, a bHLH transcriptional gene, defines GABAergic neuronal fates in cerebellum. Neuron. 2005 Jul 21;47(2):201-213. https://doi.org/10.1016/j.neuron.2005.06.007
Hoshino, Mikio ; Nakamura, Shoko ; Mori, Kiyoshi ; Kawauchi, Takeshi ; Terao, Mami ; Nishimura, Yoshiaki V. ; Fukuda, Akihisa ; Fuse, Toshimitsu ; Matsuo, Naoki ; Sone, Masaki ; Watanabe, Masahiko ; Bito, Haruhiko ; Terashima, Toshio ; Wright, Christopher V E ; Kawaguchi, Yoshiya ; Nakao, Kazuwa ; Nabeshima, Yo Ichi. / Ptf1a, a bHLH transcriptional gene, defines GABAergic neuronal fates in cerebellum. In: Neuron. 2005 ; Vol. 47, No. 2. pp. 201-213.
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