PTH-related protein enhances LoVo colon cancer cell proliferation, adhesion, and integrin expression

Xiaoli Shen, Miriam Falzon

Research output: Contribution to journalArticle

27 Scopus citations

Abstract

Parathyroid hormone-related protein (PTHrP) has been localized in human colon cancer tissue and cell lines. Tumor cell adhesion to extracellular matrix (ECM) proteins plays a major role in the invasion and metastasis of tumor cells, and is mediated via integrin subunits. The LoVo human colon cancer cell line was used as a model system to study the effects of PTHrP on cell proliferation and adhesion to ECM proteins found in normal liver. Clones of LoVo cells engineered to overexpress PTHrP by stable transfection with a PTHrP cDNA showed enhanced cell proliferation vs. control (empty vector-transfected) cells. PTHrP-overexpressing cells also showed significantly higher adhesion to collagen type I, fibronectin, and laminin, and enhanced expression of the ∀2, ∀5, ∀6, ∃1 and ∃4 integrin subunits. These results indicate that PTHrP may play a role in colon cancer invasion and metastasis by increasing cell proliferation and adhesion to the ECM via upregulation of proinvasive integrin expression.

Original languageEnglish (US)
Pages (from-to)17-27
Number of pages11
JournalRegulatory Peptides
Volume125
Issue number1-3
DOIs
StatePublished - Feb 15 2005
Externally publishedYes

Keywords

  • Adhesion
  • Extracellular matrix
  • Integrins
  • LoVo (colon cancer cells)
  • Parathyroid hormone-related protein
  • Proliferation

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Endocrinology
  • Clinical Biochemistry
  • Cellular and Molecular Neuroscience

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