PTH-related protein modulates PC-3 prostate cancer cell adhesion and integrin subunit profile

Xiaoli Shen, Miriam Falzon

Research output: Contribution to journalArticle

48 Scopus citations

Abstract

Parathyroid hormone-related protein (PTHrP), which has been localized in prostate cancer tissue and cell lines, plays a role in the development of bone metastases, a frequent complication in prostate cancer patients. Tumor cell adhesion to extracellular matrix (ECM) components is mediated via integrin subunits, and plays a major role in the invasion and metastasis of tumor cells. The present experiments examined the ability of PTHrP to influence adhesion of the human prostate cancer cell line PC-3 to several ECM proteins found in normal tissues. Clonal PC-3 cells induced to overexpress PTHrP by stable transfection with PTHrP complementary DNA showed significantly higher adhesion to collagen type 1, fibronectin, and laminin than control (empty vector-transfected) cells. PTHrP-overexpressing cells also exhibited higher expression of the α1, α5, α6, and β4 integrin subunits. These results suggest that PTHrP may play a role in prostate tumor invasion and metastasis by influencing cell adhesion to the ECM via upregulation of specific integrin subunits.

Original languageEnglish (US)
Pages (from-to)165-177
Number of pages13
JournalMolecular and Cellular Endocrinology
Volume199
Issue number1-2
DOIs
StatePublished - Jan 31 2003

Keywords

  • Extracellular matrix
  • Integrins
  • PC-3 (prostate cancer cells)
  • Parathyroid hormone-related protein

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Endocrinology

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