PU.1 regulates the CXCR1 promoter

Nancy C. Wilkinson, Javier Navarro

    Research output: Contribution to journalArticlepeer-review

    13 Scopus citations

    Abstract

    The interleukin-8 receptors (CXCR1 and CXCR2) are specifically expressed at high levels in cells of the neutrophil lineage. In this work we identify promoter elements of the CXCR1 gene and the ets family transcription factor PU.1 as a major regulator for activation of the CXCR1 promoter. We first showed that the upstream sequence of CXCR1 (-800 to +86 base pairs (bp)) directs myeloid-specific expression of reporter gene constructs. Second, we showed the presence of negative elements in the sequence from -800 to -128 bp and positive elements from -128 to + 50 bp. Third, we demonstrated that the fragment -22 to +14 bp binds PU. 1. Fourth, we showed that PU.1 transactivates the CXCR1 promoter. These data are the first demonstration of PU.1-mediated transcriptional regulation of a neutrophil chemoattractant G protein-coupled receptor.

    Original languageEnglish (US)
    Pages (from-to)438-443
    Number of pages6
    JournalJournal of Biological Chemistry
    Volume274
    Issue number1
    DOIs
    StatePublished - Jan 1 1999

    ASJC Scopus subject areas

    • Biochemistry
    • Molecular Biology
    • Cell Biology

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