Pulmonary changes in a mouse model of combined burn and smoke inhalation-induced injury

Akio Mizutani, Perenlei Enkhbaatar, Aimalohi Esechie, Lillian D. Traber, Robert A. Cox, Hal K. Hawkins, Donald J. Deyo, Kazunori Murakami, Takayuki Noguchi, Daniel L. Traber

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

The morbidity and mortality of burn victims increase when burn injury is combined with smoke inhalation. The goal of the present study was to develop a murine model of burn and smoke inhalation injury to more precisely reveal the mechanistic aspects of these pathological changes. The burn injury mouse group received a 40% total body surface area third-degree burn alone, the smoke inhalation injury mouse group received two 30-s exposures of cotton smoke alone, and the combined burn and smoke inhalation injury mouse group received both the burn and the smoke inhalation injury. Animal survival was monitored for 120 h. Survival rates in the burn injury group, the smoke inhalation injury group, and the combined injury group were 70%, 60%, and 30%, respectively. Mice that received combined burn and smoke injury developed greater lung damage as evidenced by histological changes (septal thickening and interstitial edema) and higher lung water content. These mice also displayed more severely impaired pulmonary gas exchange [arterial PO2 (PaO2)/inspired O2 fraction (FIO2) < 200]. Lung myeloperoxidase activity was significantly higher in burn and smoke-injured animals compared with the other three experimental groups. Plasma NO2 -/NO3-, lung inducible nitric oxide synthase (iNOS) activity, and iNOS mRNA increased with injury; however, the burn and smoke injury group exhibited a higher response. Severity of burn and smoke inhalation injury was associated with more pronounced production of nitric oxide and accumulation of activated leukocytes in lung tissue. The murine model of burn and smoke inhalation injury allows us to better understand pathophysiological mechanisms underlying cardiopulmonary morbidity secondary to burn and smoke inhalation injury.

Original languageEnglish (US)
Pages (from-to)678-684
Number of pages7
JournalJournal of Applied Physiology
Volume105
Issue number2
DOIs
StatePublished - Aug 1 2008

    Fingerprint

Keywords

  • Acute lung injury
  • Inducible nitric oxide synthase
  • Nitric oxide

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

Mizutani, A., Enkhbaatar, P., Esechie, A., Traber, L. D., Cox, R. A., Hawkins, H. K., Deyo, D. J., Murakami, K., Noguchi, T., & Traber, D. L. (2008). Pulmonary changes in a mouse model of combined burn and smoke inhalation-induced injury. Journal of Applied Physiology, 105(2), 678-684. https://doi.org/10.1152/japplphysiol.00232.2007