Pulmonary expression of nitric oxide synthase isoforms in sheep with smoke inhalation and burn injury

Robert A. Cox, Sam Jacob, Gloria Oliveras, Kazunori Murakami, Perenlei Enkhbaatar, Lillian Traber, Frank C. Schmalstieg, David N. Herndon, Daniel L. Traber, Hal K. Hawkins

Research output: Contribution to journalArticlepeer-review

30 Scopus citations


Previous studies have indicated increased plasma levels of inducible nitric oxide synthase in lung. This study further examines the pulmonary expression of nitric oxide synthase (NOS) isoforms in an ovine model of acute lung injury induced by smoke inhalation and burn injury (S+B injury). Female range bred sheep (4 per group) were sacrificed at 4, 8, 12, 24, and 48 hours after injury and immunohistochemistry was performed in tissues for various NOS isoforms. The study indicates that in uninjured sheep lung, endothelial (eNOS) is constitutively expressed in the endothelial cells associated with the airways and parenchyma, and in macrophages. Similarly, neuronal (nNOS) is constitutively present in the mucous cells of the epithelium and in neurons of airway ganglia. In uninjured lung, inducible (iNOS) was present in bronchial secretory cells and macrophages. In tissue after S+B injury, new expression of iNOS was evident in bronchial ciliated cells, basal cells, and mucus gland cells. In the parenchyma, a slight increase in iNOS immunostaining was seen in type I cells at 12 and 24 hours after injury only. Virtually no change in eNOS or nNOS was seen after injury.

Original languageEnglish (US)
Pages (from-to)104-118
Number of pages15
JournalExperimental Lung Research
Issue number2
StatePublished - Mar 2009


  • Acute lung injury
  • Airway
  • Immunohistochemistry
  • Lung
  • Mucous glands
  • Nitric oxide
  • Nitric oxide isoforms
  • Sheep

ASJC Scopus subject areas

  • Molecular Biology
  • Pulmonary and Respiratory Medicine
  • Clinical Biochemistry


Dive into the research topics of 'Pulmonary expression of nitric oxide synthase isoforms in sheep with smoke inhalation and burn injury'. Together they form a unique fingerprint.

Cite this