Pulmonary inflammatory cell response to sustained endotoxin administration

C. Z. Wang, David Herndon, L. D. Traber, S. F. Yang, R. A. Cox, H. Nakazawa, R. E. Barrow, D. L. Traber

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

We have developed a model of human sepsis in sheep. Twenty-four hours after continuous infusion of Escherichia coli endotoxin (lipopolysaccharide) (10 ng · kg-1 · min-1) was begun, pulmonary transvascular fluid flux was almost five times the baseline values, cardiac output was nearly doubled, and mean arterial pressure was reduced by ~20 mmHg. At this time, the animals were killed and their lungs were fixed by endotracheal installation of 2.5% glutaraldehyde at 25 cmH2O pressure. Morphometry was performed by point counting, and data were expressed as relative volume density. Pulmonary edema and congestion were observed in sheep receiving lipopolysaccharide, whereas sham controls appeared normal. There was an increase in interstitial volume density. There was a significant increase (P < 0.01) in volume density of the pulmonary intravasculature (180%), interstitial macrophages (270%), and mast cells (240%). The volume densities of intravascular and interstitial polymorphonuclear neutrophils also showed a small insignificant increase.

Original languageEnglish (US)
Pages (from-to)516-522
Number of pages7
JournalJournal of Applied Physiology
Volume76
Issue number2
StatePublished - 1994

Fingerprint

Endotoxins
Lung
Lipopolysaccharides
Sheep
Specific Gravity
Glutaral
Pulmonary Edema
Mast Cells
Cardiac Output
Sepsis
Arterial Pressure
Neutrophils
Macrophages
Pressure
Escherichia coli endotoxin

Keywords

  • lipopolysaccharide macrophages
  • lung injury
  • morphometry
  • sepsis

ASJC Scopus subject areas

  • Endocrinology
  • Physiology
  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Wang, C. Z., Herndon, D., Traber, L. D., Yang, S. F., Cox, R. A., Nakazawa, H., ... Traber, D. L. (1994). Pulmonary inflammatory cell response to sustained endotoxin administration. Journal of Applied Physiology, 76(2), 516-522.

Pulmonary inflammatory cell response to sustained endotoxin administration. / Wang, C. Z.; Herndon, David; Traber, L. D.; Yang, S. F.; Cox, R. A.; Nakazawa, H.; Barrow, R. E.; Traber, D. L.

In: Journal of Applied Physiology, Vol. 76, No. 2, 1994, p. 516-522.

Research output: Contribution to journalArticle

Wang, CZ, Herndon, D, Traber, LD, Yang, SF, Cox, RA, Nakazawa, H, Barrow, RE & Traber, DL 1994, 'Pulmonary inflammatory cell response to sustained endotoxin administration', Journal of Applied Physiology, vol. 76, no. 2, pp. 516-522.
Wang CZ, Herndon D, Traber LD, Yang SF, Cox RA, Nakazawa H et al. Pulmonary inflammatory cell response to sustained endotoxin administration. Journal of Applied Physiology. 1994;76(2):516-522.
Wang, C. Z. ; Herndon, David ; Traber, L. D. ; Yang, S. F. ; Cox, R. A. ; Nakazawa, H. ; Barrow, R. E. ; Traber, D. L. / Pulmonary inflammatory cell response to sustained endotoxin administration. In: Journal of Applied Physiology. 1994 ; Vol. 76, No. 2. pp. 516-522.
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