Pulmonary microvascular hyperpermeability and expression of vascular endothelial growth factor in smoke inhalation- and pneumonia-induced acute lung injury

Matthias Lange, Atsumori Hamahata, Daniel L. Traber, Rhykka Connelly, Yoshimitsu Nakano, Lillian D. Traber, Frank C. Schmalstieg, David Herndon, Perenlei Enkhbaatar

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Introduction: Acute lung injury (ALI) and sepsis are major contributors to the morbidity and mortality of critically ill patients. The current study was designed further evaluate the mechanism of pulmonary vascular hyperpermeability in sheep with these injuries. Methods: Sheep were randomized to a sham-injured control group (n = 6) or ALI/sepsis group (n = 7). The sheep in the ALI/sepsis group received inhalation injury followed by instillation of Pseudomonas aeruginosa into the lungs. These groups were monitored for 24 h. Additional sheep (n = 16) received the injury and lung tissue was harvested at different time points to measure lung wet/dry weight ratio, vascular endothelial growth factor (VEGF) mRNA and protein expression as well as 3-nitrotyrosine protein expression in lung homogenates. Results: The injury induced severe deterioration in pulmonary gas exchange, increases in lung lymph flow and protein content, and lung water content (P < 0.01 each). These alterations were associated with elevated lung and plasma nitrite/nitrate concentrations, increased tracheal blood flow, and enhanced VEGF mRNA and protein expression in lung tissue as well as enhanced 3-nitrotyrosine protein expression (P < 0.05 each). Conclusions: This study describes the time course of pulmonary microvascular hyperpermeability in a clinical relevant large animal model and may improve the experimental design of future studies.

Original languageEnglish (US)
Pages (from-to)1072-1078
Number of pages7
JournalBurns
Volume38
Issue number7
DOIs
StatePublished - Nov 2012

Fingerprint

Acute Lung Injury
Smoke
Inhalation
Vascular Endothelial Growth Factor A
Pneumonia
Lung
Sheep
Sepsis
Proteins
Wounds and Injuries
Pulmonary Gas Exchange
Messenger RNA
Lung Injury
Lymph
Pulmonary Edema
Nitrites
Critical Illness
Nitrates
Pseudomonas aeruginosa
Blood Vessels

Keywords

  • Microvascular hyperpermeability
  • Pulmonary edema
  • Transvascular fluid flux
  • Vascular endothelial growth factor

ASJC Scopus subject areas

  • Emergency Medicine
  • Critical Care and Intensive Care Medicine
  • Surgery

Cite this

Pulmonary microvascular hyperpermeability and expression of vascular endothelial growth factor in smoke inhalation- and pneumonia-induced acute lung injury. / Lange, Matthias; Hamahata, Atsumori; Traber, Daniel L.; Connelly, Rhykka; Nakano, Yoshimitsu; Traber, Lillian D.; Schmalstieg, Frank C.; Herndon, David; Enkhbaatar, Perenlei.

In: Burns, Vol. 38, No. 7, 11.2012, p. 1072-1078.

Research output: Contribution to journalArticle

Lange, Matthias ; Hamahata, Atsumori ; Traber, Daniel L. ; Connelly, Rhykka ; Nakano, Yoshimitsu ; Traber, Lillian D. ; Schmalstieg, Frank C. ; Herndon, David ; Enkhbaatar, Perenlei. / Pulmonary microvascular hyperpermeability and expression of vascular endothelial growth factor in smoke inhalation- and pneumonia-induced acute lung injury. In: Burns. 2012 ; Vol. 38, No. 7. pp. 1072-1078.
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