Pulmonary toxicity of acrylonitrile

covalent interaction and effect on replicative and unscheduled DNA synthesis in the lung

Ahmed E. Ahmed, A. H. Abdel-Aziz, Sherif Abdel-Rahman, Abida K. Haque, Amr M. Nouraldeen, Samia A. Shouman

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Acrylonitrile (VCN)-induced lung toxicity was studied following a single oral dose (46.5 mg/kg). The mechanism of toxic injury was investigated by assessing the covalent interaction of [2,3-14C]VCN with pulmonary DNA. The effect of the same dose on replicative DNA synthesis and repair in the lungs of treated rats was also investigated. Histologic examination revealed that lungs of VCN-treated animals showed moderate to marked hyperplasia of the Clara cells lining the bronchioles. [14C]Lung tissue uptake was extremely fast, having a maximum at 0.5 h after treatment (150 DPM/mg tissue). Radioactivity in lung tissue declined gradually as a function of time, but was still detected at 72 h after treatment (59 DPM/mg tissue). Covalent binding of [14C]VCN-derived radioactivity to pulmonary DNA was time-dependent, reaching a maximum at 12 h following treatment (61 DPM/mg DNA) and was still detected at 72 h (27 DPM/mg DNA) indicating the incomplete removal of radioactivity covalently bound to DNA. Replicative DNA synthesis in lung tissue was significantly decreased at all time points studied (59, 55 and 72% of control at 0.5, 6 and 24 h, respectively). The DNA repair in the lung was increased by 2-fold at 0.5 h and 1.6-fold at 6 h following VCN oral treatment. This histologic and biochemical results presented in this study provide evidence for the acute genetic toxicity of VCN (and/or its metabolites) in lung tissue following a single oral dose of VCN.

Original languageEnglish (US)
Pages (from-to)1-14
Number of pages14
JournalToxicology
Volume76
Issue number1
DOIs
StatePublished - Nov 22 1992

Fingerprint

Acrylonitrile
Toxicity
Lung
DNA
Tissue
Radioactivity
Repair
DNA Repair
Poisons
Bronchioles
Metabolites
Linings
Rats
Animals
Hyperplasia

Keywords

  • Acrylonitrile
  • Covalent binding
  • Genotoxicity
  • Lung
  • Replicative DNA synthesis
  • Unscheduled DNA synthesis

ASJC Scopus subject areas

  • Toxicology

Cite this

Pulmonary toxicity of acrylonitrile : covalent interaction and effect on replicative and unscheduled DNA synthesis in the lung. / Ahmed, Ahmed E.; Abdel-Aziz, A. H.; Abdel-Rahman, Sherif; Haque, Abida K.; Nouraldeen, Amr M.; Shouman, Samia A.

In: Toxicology, Vol. 76, No. 1, 22.11.1992, p. 1-14.

Research output: Contribution to journalArticle

Ahmed, Ahmed E. ; Abdel-Aziz, A. H. ; Abdel-Rahman, Sherif ; Haque, Abida K. ; Nouraldeen, Amr M. ; Shouman, Samia A. / Pulmonary toxicity of acrylonitrile : covalent interaction and effect on replicative and unscheduled DNA synthesis in the lung. In: Toxicology. 1992 ; Vol. 76, No. 1. pp. 1-14.
@article{c460e5184d05471cae424c95f22ef7bc,
title = "Pulmonary toxicity of acrylonitrile: covalent interaction and effect on replicative and unscheduled DNA synthesis in the lung",
abstract = "Acrylonitrile (VCN)-induced lung toxicity was studied following a single oral dose (46.5 mg/kg). The mechanism of toxic injury was investigated by assessing the covalent interaction of [2,3-14C]VCN with pulmonary DNA. The effect of the same dose on replicative DNA synthesis and repair in the lungs of treated rats was also investigated. Histologic examination revealed that lungs of VCN-treated animals showed moderate to marked hyperplasia of the Clara cells lining the bronchioles. [14C]Lung tissue uptake was extremely fast, having a maximum at 0.5 h after treatment (150 DPM/mg tissue). Radioactivity in lung tissue declined gradually as a function of time, but was still detected at 72 h after treatment (59 DPM/mg tissue). Covalent binding of [14C]VCN-derived radioactivity to pulmonary DNA was time-dependent, reaching a maximum at 12 h following treatment (61 DPM/mg DNA) and was still detected at 72 h (27 DPM/mg DNA) indicating the incomplete removal of radioactivity covalently bound to DNA. Replicative DNA synthesis in lung tissue was significantly decreased at all time points studied (59, 55 and 72{\%} of control at 0.5, 6 and 24 h, respectively). The DNA repair in the lung was increased by 2-fold at 0.5 h and 1.6-fold at 6 h following VCN oral treatment. This histologic and biochemical results presented in this study provide evidence for the acute genetic toxicity of VCN (and/or its metabolites) in lung tissue following a single oral dose of VCN.",
keywords = "Acrylonitrile, Covalent binding, Genotoxicity, Lung, Replicative DNA synthesis, Unscheduled DNA synthesis",
author = "Ahmed, {Ahmed E.} and Abdel-Aziz, {A. H.} and Sherif Abdel-Rahman and Haque, {Abida K.} and Nouraldeen, {Amr M.} and Shouman, {Samia A.}",
year = "1992",
month = "11",
day = "22",
doi = "10.1016/0300-483X(92)90013-5",
language = "English (US)",
volume = "76",
pages = "1--14",
journal = "Toxicology",
issn = "0300-483X",
publisher = "Elsevier Ireland Ltd",
number = "1",

}

TY - JOUR

T1 - Pulmonary toxicity of acrylonitrile

T2 - covalent interaction and effect on replicative and unscheduled DNA synthesis in the lung

AU - Ahmed, Ahmed E.

AU - Abdel-Aziz, A. H.

AU - Abdel-Rahman, Sherif

AU - Haque, Abida K.

AU - Nouraldeen, Amr M.

AU - Shouman, Samia A.

PY - 1992/11/22

Y1 - 1992/11/22

N2 - Acrylonitrile (VCN)-induced lung toxicity was studied following a single oral dose (46.5 mg/kg). The mechanism of toxic injury was investigated by assessing the covalent interaction of [2,3-14C]VCN with pulmonary DNA. The effect of the same dose on replicative DNA synthesis and repair in the lungs of treated rats was also investigated. Histologic examination revealed that lungs of VCN-treated animals showed moderate to marked hyperplasia of the Clara cells lining the bronchioles. [14C]Lung tissue uptake was extremely fast, having a maximum at 0.5 h after treatment (150 DPM/mg tissue). Radioactivity in lung tissue declined gradually as a function of time, but was still detected at 72 h after treatment (59 DPM/mg tissue). Covalent binding of [14C]VCN-derived radioactivity to pulmonary DNA was time-dependent, reaching a maximum at 12 h following treatment (61 DPM/mg DNA) and was still detected at 72 h (27 DPM/mg DNA) indicating the incomplete removal of radioactivity covalently bound to DNA. Replicative DNA synthesis in lung tissue was significantly decreased at all time points studied (59, 55 and 72% of control at 0.5, 6 and 24 h, respectively). The DNA repair in the lung was increased by 2-fold at 0.5 h and 1.6-fold at 6 h following VCN oral treatment. This histologic and biochemical results presented in this study provide evidence for the acute genetic toxicity of VCN (and/or its metabolites) in lung tissue following a single oral dose of VCN.

AB - Acrylonitrile (VCN)-induced lung toxicity was studied following a single oral dose (46.5 mg/kg). The mechanism of toxic injury was investigated by assessing the covalent interaction of [2,3-14C]VCN with pulmonary DNA. The effect of the same dose on replicative DNA synthesis and repair in the lungs of treated rats was also investigated. Histologic examination revealed that lungs of VCN-treated animals showed moderate to marked hyperplasia of the Clara cells lining the bronchioles. [14C]Lung tissue uptake was extremely fast, having a maximum at 0.5 h after treatment (150 DPM/mg tissue). Radioactivity in lung tissue declined gradually as a function of time, but was still detected at 72 h after treatment (59 DPM/mg tissue). Covalent binding of [14C]VCN-derived radioactivity to pulmonary DNA was time-dependent, reaching a maximum at 12 h following treatment (61 DPM/mg DNA) and was still detected at 72 h (27 DPM/mg DNA) indicating the incomplete removal of radioactivity covalently bound to DNA. Replicative DNA synthesis in lung tissue was significantly decreased at all time points studied (59, 55 and 72% of control at 0.5, 6 and 24 h, respectively). The DNA repair in the lung was increased by 2-fold at 0.5 h and 1.6-fold at 6 h following VCN oral treatment. This histologic and biochemical results presented in this study provide evidence for the acute genetic toxicity of VCN (and/or its metabolites) in lung tissue following a single oral dose of VCN.

KW - Acrylonitrile

KW - Covalent binding

KW - Genotoxicity

KW - Lung

KW - Replicative DNA synthesis

KW - Unscheduled DNA synthesis

UR - http://www.scopus.com/inward/record.url?scp=0026480664&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026480664&partnerID=8YFLogxK

U2 - 10.1016/0300-483X(92)90013-5

DO - 10.1016/0300-483X(92)90013-5

M3 - Article

VL - 76

SP - 1

EP - 14

JO - Toxicology

JF - Toxicology

SN - 0300-483X

IS - 1

ER -