Pulmonary vascular permeability changes in an ovine model of methicillin-resistant Staphylococcus aureus sepsis

Collette C. Jonkam, Kamna Bansal, Daniel L. Traber, Atsumori Hamahata, Marc O. Maybauer, Dirk M. Maybauer, Robert A. Cox, Matthias Lange, Rhykka L. Connelly, Lillian D. Traber, Clarisse D. Djukom, John R. Salsbury, David Herndon, Perenlei Enkhbaatar

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Introduction: Endothelial dysfunction is a hallmark of sepsis, associated with lung transvascular fluid flux and pulmonary dysfunction in septic patients. We tested the hypothesis that methicillin-resistant Staphylococcus aureus (MRSA) sepsis following smoke inhalation increases pulmonary transvascular fluid flux via excessive nitric oxide (NO) production. Methods: Ewes were chronically instrumented, and randomised into either a control or MRSA sepsis (MRSA and smoke inhalation) group. Results: Pulmonary function remained stable in the control group, whereas the MRSA sepsis group developed impaired gas exchange and significantly increased lung lymph flow, permeability index and bloodless wet-to-dry weight-ratio (W/D ratio). The plasma nitrate/ nitrite (NOx) levels, lung inducible nitric oxide synthases (iNOS) and endothelial nitric oxide synthases (eNOS), vascular endothelial growth factor (VEGF) protein expressions and poly-(ADP)-ribose (PAR) were significantly increased by MRSA challenge. Conclusions: These results provide evidence that excessive NO production may mediate pulmonary vascular hyperpermeability in MRSA sepsis via up regulation of reactive radicals and VEGF.

Original languageEnglish (US)
Article numberR19
JournalCritical Care
Volume13
Issue number1
DOIs
StatePublished - Feb 17 2009

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Capillary Permeability
Methicillin-Resistant Staphylococcus aureus
Sheep
Sepsis
Lung
Smoke
Inhalation
Vascular Endothelial Growth Factor A
Nitric Oxide
Poly Adenosine Diphosphate Ribose
Nitric Oxide Synthase Type III
Lymph
Nitric Oxide Synthase Type II
Nitrites
Nitrates
Blood Vessels
Permeability
Up-Regulation
Gases
Weights and Measures

ASJC Scopus subject areas

  • Critical Care and Intensive Care Medicine
  • Medicine(all)

Cite this

Pulmonary vascular permeability changes in an ovine model of methicillin-resistant Staphylococcus aureus sepsis. / Jonkam, Collette C.; Bansal, Kamna; Traber, Daniel L.; Hamahata, Atsumori; Maybauer, Marc O.; Maybauer, Dirk M.; Cox, Robert A.; Lange, Matthias; Connelly, Rhykka L.; Traber, Lillian D.; Djukom, Clarisse D.; Salsbury, John R.; Herndon, David; Enkhbaatar, Perenlei.

In: Critical Care, Vol. 13, No. 1, R19, 17.02.2009.

Research output: Contribution to journalArticle

Jonkam, CC, Bansal, K, Traber, DL, Hamahata, A, Maybauer, MO, Maybauer, DM, Cox, RA, Lange, M, Connelly, RL, Traber, LD, Djukom, CD, Salsbury, JR, Herndon, D & Enkhbaatar, P 2009, 'Pulmonary vascular permeability changes in an ovine model of methicillin-resistant Staphylococcus aureus sepsis', Critical Care, vol. 13, no. 1, R19. https://doi.org/10.1186/cc7720
Jonkam CC, Bansal K, Traber DL, Hamahata A, Maybauer MO, Maybauer DM et al. Pulmonary vascular permeability changes in an ovine model of methicillin-resistant Staphylococcus aureus sepsis. Critical Care. 2009 Feb 17;13(1). R19. https://doi.org/10.1186/cc7720
Jonkam, Collette C. ; Bansal, Kamna ; Traber, Daniel L. ; Hamahata, Atsumori ; Maybauer, Marc O. ; Maybauer, Dirk M. ; Cox, Robert A. ; Lange, Matthias ; Connelly, Rhykka L. ; Traber, Lillian D. ; Djukom, Clarisse D. ; Salsbury, John R. ; Herndon, David ; Enkhbaatar, Perenlei. / Pulmonary vascular permeability changes in an ovine model of methicillin-resistant Staphylococcus aureus sepsis. In: Critical Care. 2009 ; Vol. 13, No. 1.
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