Pulsed-light imaging for fluorescence guided surgery under normal room lighting

Kristian Sexton, Scott C. Davis, David McClatchy, Pablo A. Valdes, Stephen C. Kanick, Keith D. Paulsen, David W. Roberts, Brian W. Pogue

Research output: Contribution to journalArticlepeer-review

42 Scopus citations


Fluorescence guided surgery (FGS) is an emerging technology that has demonstrated improved surgical outcomes. However, dim lighting conditions required by current FGS systems are disruptive to standard surgical workflow. We present a novel FGS system capable of imaging fluorescence under normal room light by using pulsed excitation and gated acquisition. Images from tissue-simulating phantoms confirm visual detection down to 0.25 μM of protoporphyrin IX under 125 μW/cm2 of ambient light, more than an order of magnitude lower than that measured with the Zeiss Pentero in the dark. Resection of orthotopic brain tumors in mice also suggests that the pulsed-light system provides superior sensitivity in vivo.

Original languageEnglish (US)
Pages (from-to)3249-3252
Number of pages4
JournalOptics Letters
Issue number17
StatePublished - Sep 1 2013
Externally publishedYes

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics


Dive into the research topics of 'Pulsed-light imaging for fluorescence guided surgery under normal room lighting'. Together they form a unique fingerprint.

Cite this