Pump-probe imaging of pigmented cutaneous melanoma primary lesions gives insight into metastatic potential

Francisco E. Robles, Sanghamitra Deb, Jesse W. Wilson, Christina S. Gainey, M. Angelica Selim, Paul J. Mosca, Douglas Tyler, Martin C. Fischer, Warren S. Warren

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Metastatic melanoma is associated with a poor prognosis, but no method reliably predicts which melanomas of a given stage will ultimately metastasize and which will not. While sentinel lymph node biopsy (SLNB) has emerged as the most powerful predictor of metastatic disease, the majority of people dying from metastatic melanoma still have a negative SLNB. Here we analyze pump-probe microscopy images of thin biopsy slides of primary melanomas to assess their metastatic potential. Pumpprobe microscopy reveals detailed chemical information of melanin with subcellular spatial resolution. Quantification of the molecular signatures without reference standards is achieved using a geometrical representation of principal component analysis. Melanin structure is analyzed in unison with the chemical information by applying principles of mathematical morphology. Results show that melanin in metastatic primary lesions has lower chemical diversity than non-metastatic primary lesions, and contains two distinct phenotypes that are indicative of aggressive disease. Further, the mathematical morphology analysis reveals melanin in metastatic primary lesions has a distinct “dusty” quality. Finally, a statistical analysis shows that the combination of the chemical information with spatial structures predicts metastatic potential with much better sensitivity than SLNB and high specificity, suggesting pump-probe microscopy can be an important tool to help predict the metastatic potential of melanomas.

Original languageEnglish (US)
Article numberA026
Pages (from-to)3631-3645
Number of pages15
JournalBiomedical Optics Express
Volume6
Issue number9
DOIs
StatePublished - 2015

Fingerprint

melanin
lesions
Melanins
lymphatic system
Melanoma
Sentinel Lymph Node Biopsy
pumps
Skin
probes
Microscopy
microscopy
phenotype
prognosis
principal components analysis
chutes
statistical analysis
Principal Component Analysis
spatial resolution
signatures
sensitivity

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Biotechnology

Cite this

Robles, F. E., Deb, S., Wilson, J. W., Gainey, C. S., Selim, M. A., Mosca, P. J., ... Warren, W. S. (2015). Pump-probe imaging of pigmented cutaneous melanoma primary lesions gives insight into metastatic potential. Biomedical Optics Express, 6(9), 3631-3645. [A026]. https://doi.org/10.1364/BOE.6.00363

Pump-probe imaging of pigmented cutaneous melanoma primary lesions gives insight into metastatic potential. / Robles, Francisco E.; Deb, Sanghamitra; Wilson, Jesse W.; Gainey, Christina S.; Selim, M. Angelica; Mosca, Paul J.; Tyler, Douglas; Fischer, Martin C.; Warren, Warren S.

In: Biomedical Optics Express, Vol. 6, No. 9, A026, 2015, p. 3631-3645.

Research output: Contribution to journalArticle

Robles, FE, Deb, S, Wilson, JW, Gainey, CS, Selim, MA, Mosca, PJ, Tyler, D, Fischer, MC & Warren, WS 2015, 'Pump-probe imaging of pigmented cutaneous melanoma primary lesions gives insight into metastatic potential', Biomedical Optics Express, vol. 6, no. 9, A026, pp. 3631-3645. https://doi.org/10.1364/BOE.6.00363
Robles, Francisco E. ; Deb, Sanghamitra ; Wilson, Jesse W. ; Gainey, Christina S. ; Selim, M. Angelica ; Mosca, Paul J. ; Tyler, Douglas ; Fischer, Martin C. ; Warren, Warren S. / Pump-probe imaging of pigmented cutaneous melanoma primary lesions gives insight into metastatic potential. In: Biomedical Optics Express. 2015 ; Vol. 6, No. 9. pp. 3631-3645.
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