Purification and characterization of Aplysia atrial gland secretory granules containing egg-laying prohormone-related peptides

Gregg T. Nagle, Walter R A Van Heumen, Mahdy A. El-Hamzawy, Alexander Kurosky

    Research output: Contribution to journalArticle

    8 Citations (Scopus)

    Abstract

    A purification scheme is described for the isolation of secretory granules containing egg-laying prohormone-related peptides from the atrial gland of Aplysia californica, an exocrine organ in the reproductive tract. Granules were purified by differential centrifugation of atrial gland homogenates followed by centrifugation on continuous Percoll-sucrose gradients. Quantitative enzyme assays in conjunction with electron microscopic analyses demonstrated that secretory granules thus isolated were significantly purified with respect to other subcellular organelles such as mitochondria and lysosomes. Immunoelectron microscopy demonstrated that the majority (∼85%) of the purified secretory granules were immunoreactive for A-NTP (N-terminal peptide), a cleavage product of the egg-laying prohormone-related A and A′ precursors (residues 22-34). The purified granules represented an enriched source of peptides that were readily resolved by reversed-phase high performance liquid chromatography.

    Original languageEnglish (US)
    Pages (from-to)101-108
    Number of pages8
    JournalPeptides
    Volume15
    Issue number1
    DOIs
    StatePublished - 1994

    Fingerprint

    Aplysia
    Centrifugation
    Secretory Vesicles
    Purification
    Ovum
    Peptides
    Mitochondria
    High performance liquid chromatography
    Sucrose
    Assays
    Microscopic examination
    Immunoelectron Microscopy
    Enzyme Assays
    Reverse-Phase Chromatography
    Lysosomes
    Organelles
    Electrons
    Enzymes
    High Pressure Liquid Chromatography
    Percoll

    Keywords

    • Aplysia californica
    • Atrial gland
    • Egg-laying hormone gene family

    ASJC Scopus subject areas

    • Biochemistry
    • Endocrinology
    • Physiology
    • Cellular and Molecular Neuroscience

    Cite this

    Purification and characterization of Aplysia atrial gland secretory granules containing egg-laying prohormone-related peptides. / Nagle, Gregg T.; Van Heumen, Walter R A; El-Hamzawy, Mahdy A.; Kurosky, Alexander.

    In: Peptides, Vol. 15, No. 1, 1994, p. 101-108.

    Research output: Contribution to journalArticle

    Nagle, Gregg T. ; Van Heumen, Walter R A ; El-Hamzawy, Mahdy A. ; Kurosky, Alexander. / Purification and characterization of Aplysia atrial gland secretory granules containing egg-laying prohormone-related peptides. In: Peptides. 1994 ; Vol. 15, No. 1. pp. 101-108.
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    AU - Kurosky, Alexander

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