Purification and characterization of glutathione S-transferase from sugarcane leaves

Sharad S. Singhal, Narendra K. Tiwari, Hassan Ahmad, Satish Srivastava, Yogesh C. Awasthi

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Glutathione S-transferase has been purified from leaves of sugarcane using (NH4)2SO4 fractionation, isoelectric focusing, Sephadex G-100 gel filtration, DEAE cellulose ion-exchange chromatography, and affinity chromatography over GSH-linked to epoxy-activated Sepharose 6B. The enzyme has a pI value of 4.7 and consists of two subunits having Mr values of ca 22 500 and 24 000. N-Terminal amino acid sequence analysis of both the subunits indicates a high degree of homology between these two subunits. Both subunits also have primary structure homologies with the corn GST subunits characterized previously. Kinetic properties of the enzyme are described and compared with other plant glutathione S-transferases. Similar to human GST isoenzymes, sugarcane leaf GST is also inhibited by the herbicides 2,4-dichlorophenoxyacetate, 2,4,5-trichlorophenoxyacetate, and chenodeoxycholate.

Original languageEnglish (US)
Pages (from-to)1409-1414
Number of pages6
JournalPhytochemistry
Volume30
Issue number5
DOIs
StatePublished - 1991

Fingerprint

Saccharum
Glutathione Transferase
glutathione transferase
Purification
sugarcane
Chenodeoxycholic Acid
Affinity chromatography
DEAE-Cellulose Chromatography
DEAE-Cellulose
Protein Sequence Analysis
Ion Exchange Chromatography
isoelectric focusing
Isoelectric Focusing
Herbicides
Enzymes
ion exchange chromatography
affinity chromatography
Fractionation
enzymes
Chromatography

Keywords

  • glutathione
  • glutathione S-transferase.
  • Gramineae
  • Saacharum officinarum
  • sugarcane

ASJC Scopus subject areas

  • Plant Science
  • Biochemistry
  • Molecular Biology
  • Organic Chemistry
  • Drug Discovery

Cite this

Singhal, S. S., Tiwari, N. K., Ahmad, H., Srivastava, S., & Awasthi, Y. C. (1991). Purification and characterization of glutathione S-transferase from sugarcane leaves. Phytochemistry, 30(5), 1409-1414. https://doi.org/10.1016/0031-9422(91)84175-R

Purification and characterization of glutathione S-transferase from sugarcane leaves. / Singhal, Sharad S.; Tiwari, Narendra K.; Ahmad, Hassan; Srivastava, Satish; Awasthi, Yogesh C.

In: Phytochemistry, Vol. 30, No. 5, 1991, p. 1409-1414.

Research output: Contribution to journalArticle

Singhal, SS, Tiwari, NK, Ahmad, H, Srivastava, S & Awasthi, YC 1991, 'Purification and characterization of glutathione S-transferase from sugarcane leaves', Phytochemistry, vol. 30, no. 5, pp. 1409-1414. https://doi.org/10.1016/0031-9422(91)84175-R
Singhal, Sharad S. ; Tiwari, Narendra K. ; Ahmad, Hassan ; Srivastava, Satish ; Awasthi, Yogesh C. / Purification and characterization of glutathione S-transferase from sugarcane leaves. In: Phytochemistry. 1991 ; Vol. 30, No. 5. pp. 1409-1414.
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