Purification and characterization of the naturally occurring allelic variants of sn-glycerol-3-phosphate dehydrogenase in Drosophila Melanogaster

Glenn C. Bewley; David W. Niesel; Joseph R. Wilkins

doi:10.1016/0305-0491(84)90071-3

Purification and characterization of the naturally occurring allelic variants of sn-glycerol-3-phosphate dehydrogenase in Drosophila Melanogaster

Glenn C. Bewley, David W. Niesel, Joseph R. Wilkins

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

1. 1. The naturally occurring electrophoretic variants of sn-glycerol-3-phosphate dehydrogenase and a heterodimetric form of the enzyme resulting from a genetic cross of two variant strains of Drosophila were purified to homogeneity by a combination of DEAE-cellulose chromatography and 8-(6-aminohexyl)-amino-ATP-Sepharose affinity chromatography. 2. 2. Each purified protein was compared with respect to a number of physicochemical and kinetic properties. All forms of the enzyme were found to be similar, except for pI differences associated with the electrophoretic variation observed.

Original language	English (US)
Pages (from-to)	23-32
Number of pages	10
Journal	Comparative Biochemistry and Physiology -- Part B: Biochemistry and
Volume	79
Issue number	1
DOIs	https://doi.org/10.1016/0305-0491(84)90071-3
State	Published - 1984
Externally published	Yes

ASJC Scopus subject areas

Biochemistry
Physiology
Molecular Biology

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Purification and characterization of the naturally occurring allelic variants of sn-glycerol-3-phosphate dehydrogenase in Drosophila Melanogaster. / Bewley, Glenn C.; Niesel, David W.; Wilkins, Joseph R.

In: Comparative Biochemistry and Physiology -- Part B: Biochemistry and, Vol. 79, No. 1, 1984, p. 23-32.

Research output: Contribution to journal › Article › peer-review

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