Purification and crystallization of intact human rhinovirus complexed with a neutralizing fab

Thomas Smith, Elaine S. Chase

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We report the first crystallization of an intact virion, human rhinovirus 14, complexed with the Fab fragment from a neutralizing antibody. These crystals diffract to at least 6.0 Å resolution. It has been suggested that Fab's and mAb's can induce large conformational changes in the capsid upon binding. The structure of this complex should enable us to detect the existence and role of such changes.

Original languageEnglish (US)
Pages (from-to)600-606
Number of pages7
JournalVirology
Volume191
Issue number2
DOIs
StatePublished - 1992
Externally publishedYes

Fingerprint

Rhinovirus
Immunoglobulin Fab Fragments
Capsid
Crystallization
Neutralizing Antibodies
Virion

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Purification and crystallization of intact human rhinovirus complexed with a neutralizing fab. / Smith, Thomas; Chase, Elaine S.

In: Virology, Vol. 191, No. 2, 1992, p. 600-606.

Research output: Contribution to journalArticle

@article{0b17e16c434b470699f1ae8a61d4d2bc,
title = "Purification and crystallization of intact human rhinovirus complexed with a neutralizing fab",
abstract = "We report the first crystallization of an intact virion, human rhinovirus 14, complexed with the Fab fragment from a neutralizing antibody. These crystals diffract to at least 6.0 {\AA} resolution. It has been suggested that Fab's and mAb's can induce large conformational changes in the capsid upon binding. The structure of this complex should enable us to detect the existence and role of such changes.",
author = "Thomas Smith and Chase, {Elaine S.}",
year = "1992",
doi = "10.1016/0042-6822(92)90235-H",
language = "English (US)",
volume = "191",
pages = "600--606",
journal = "Virology",
issn = "0042-6822",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Purification and crystallization of intact human rhinovirus complexed with a neutralizing fab

AU - Smith, Thomas

AU - Chase, Elaine S.

PY - 1992

Y1 - 1992

N2 - We report the first crystallization of an intact virion, human rhinovirus 14, complexed with the Fab fragment from a neutralizing antibody. These crystals diffract to at least 6.0 Å resolution. It has been suggested that Fab's and mAb's can induce large conformational changes in the capsid upon binding. The structure of this complex should enable us to detect the existence and role of such changes.

AB - We report the first crystallization of an intact virion, human rhinovirus 14, complexed with the Fab fragment from a neutralizing antibody. These crystals diffract to at least 6.0 Å resolution. It has been suggested that Fab's and mAb's can induce large conformational changes in the capsid upon binding. The structure of this complex should enable us to detect the existence and role of such changes.

UR - http://www.scopus.com/inward/record.url?scp=0027099852&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027099852&partnerID=8YFLogxK

U2 - 10.1016/0042-6822(92)90235-H

DO - 10.1016/0042-6822(92)90235-H

M3 - Article

C2 - 1333115

AN - SCOPUS:0027099852

VL - 191

SP - 600

EP - 606

JO - Virology

JF - Virology

SN - 0042-6822

IS - 2

ER -