Purification and partial characterization of a soluble suppressor factor from papillomavirus-infected patients

V. Chopra, Ashok Chopra, S. Tyring

Research output: Contribution to journalArticle

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Abstract

A molecular form of soluble suppressor factor was identified in serum-free supernatant fluid of cultured peripheral blood mononuclear cells (PBMC) from patients with severe forms of papillomavirus infections (epidermodysplasia verruciformis and large treatment-resistant condyloma acuminatum). The papillomavirus-induced soluble suppressor factor (SSF-H) was highly purified by gel filtration, anion-exchange chromatography, chromatofocusing, hydrophobic chromatography, and nondissociating polyacrylamide gel electrophoresis (PAGE). The factor exhibited an apparent molecular weight of 67 kDa as determined by sodium dodecylsulfate-PAGE. The isoelectric point ranged from 4.8 to 5.2 as judged by chromatofocusing. The biologic activity of the SSF-H during purification and in subsequent characterization was examined by in vitro CTLL bioassay. Purified SSF-H was stable at 56°C for 1 h and in the pH range of 4.0 to 8.0. Complete inactivation was observed at 80°C. Trypsin and proteinase K treatment destroyed the biologic activity associated with purified SSF-H. This factor seems to be different in its molecular weight and other physicochemical properties from the known suppressor factors that affect lymphocyte functions.

Original languageEnglish (US)
Pages (from-to)249-258
Number of pages10
JournalViral Immunology
Volume4
Issue number4
StatePublished - 1991
Externally publishedYes

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Chromatography
Polyacrylamide Gel Electrophoresis
Epidermodysplasia Verruciformis
Molecular Weight
Condylomata Acuminata
Endopeptidase K
Papillomavirus Infections
Isoelectric Point
Biological Assay
Trypsin
Gel Chromatography
Anions
Blood Cells
Sodium
Lymphocytes
Therapeutics
Serum
In Vitro Techniques

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Purification and partial characterization of a soluble suppressor factor from papillomavirus-infected patients. / Chopra, V.; Chopra, Ashok; Tyring, S.

In: Viral Immunology, Vol. 4, No. 4, 1991, p. 249-258.

Research output: Contribution to journalArticle

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