Purification and properties of aldose reductase and aldehyde reductase II from human lens

B. Das, H. P. Song, Naseem Ansari, G. A. Hair, Satish Srivastava

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Aldose reductase (EC 1.1.1.21) and aldehyde reductase II (L-hexonate dehydrogenase, EC 1.1.1.2) have been purified to homogeneity from human lens by ion exchange chromatography, chromatofocusing, affinity chromatography, and Sephadex G-100 gel filtration. Aldose reductase and aldehyde reductase II are monomers of Mr 43,000 and Mr 41,000, respectively. Aldose reductase has a pI of 6.2 and the pH optimum of 6.5. Aldehyde reductase II has a pI of 5.7 and the pH optimum of 7.0. Both enzymes have a wide overlapping substrate specificity, but aldose reductase manifests a unique ability to reduce aldo-sugars to their corresponding alcohols. Aldose reductase activity is expressed with either NADH or NADPH as cofactors, whereas aldehyde reductase II is dependent on NADPH. Aldose reductase requires 0.4 M sulfate for the expression of its full activity but aldehyde reductase II is significantly inhibited by sulfate. Both enzymes are susceptible to inhibition by various aldo-keto reductase inhibitors and sulfhydryl reagent, N-ethylmyleimide, to varying degrees. Aldose reductase and aldehyde reductase II are immunologically distinct.

Original languageEnglish (US)
Pages (from-to)309-335
Number of pages27
JournalLens Research
Volume4
Issue number4
StatePublished - 1987

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L-glucuronate reductase
Aldehyde Reductase
Lenses
NADP
Sulfates
Sulfhydryl Reagents
Ion Exchange Chromatography
Enzymes
Substrate Specificity
Affinity Chromatography
NAD
Gel Chromatography

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Das, B., Song, H. P., Ansari, N., Hair, G. A., & Srivastava, S. (1987). Purification and properties of aldose reductase and aldehyde reductase II from human lens. Lens Research, 4(4), 309-335.

Purification and properties of aldose reductase and aldehyde reductase II from human lens. / Das, B.; Song, H. P.; Ansari, Naseem; Hair, G. A.; Srivastava, Satish.

In: Lens Research, Vol. 4, No. 4, 1987, p. 309-335.

Research output: Contribution to journalArticle

Das, B, Song, HP, Ansari, N, Hair, GA & Srivastava, S 1987, 'Purification and properties of aldose reductase and aldehyde reductase II from human lens', Lens Research, vol. 4, no. 4, pp. 309-335.
Das, B. ; Song, H. P. ; Ansari, Naseem ; Hair, G. A. ; Srivastava, Satish. / Purification and properties of aldose reductase and aldehyde reductase II from human lens. In: Lens Research. 1987 ; Vol. 4, No. 4. pp. 309-335.
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