Purification and properties of human kidney cortex hexosaminidases A and B

J. E. Wiktorowicz, Y. C. Awasthi, A. Kurosky, Satish Srivastava

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Hexosaminidases A and B from human kidney cortex were purified to homogeneity (concanavalin A affinity chromatography, ion-exchange chromatography and gel filtration). The yield of homogeneous isoenzymes improved about 20-fold, giving preparations of hexosaminidases A and B with specific activities of about 200 and 325 units/mg of protein respectively. The kinetic and structural properties of kidney hexosaminidase isoenzymes were studied and compared with the hexosaminidase isoenzymes from human placenta. The amino acid composition of hexosaminidase A was significantly different from that of hexosaminidase B. In the event of success in developing enzyme-replacement therapy for Tay-Sachs and Sandhoff's diseases, this modified procedure can furnish larger amounts of homogeneous isoenzymes.

Original languageEnglish (US)
Pages (from-to)49-53
Number of pages5
JournalBiochemical Journal
Volume165
Issue number1
StatePublished - 1977

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Hexosaminidase B
Hexosaminidase A
Kidney Cortex
Isoenzymes
Purification
Hexosaminidases
Sandhoff Disease
Tay-Sachs Disease
Enzyme Replacement Therapy
Affinity chromatography
Ion Exchange Chromatography
Concanavalin A
Chromatography
Affinity Chromatography
Placenta
Gel Chromatography
Structural properties
Ion exchange
Gels
Kidney

ASJC Scopus subject areas

  • Biochemistry

Cite this

Wiktorowicz, J. E., Awasthi, Y. C., Kurosky, A., & Srivastava, S. (1977). Purification and properties of human kidney cortex hexosaminidases A and B. Biochemical Journal, 165(1), 49-53.

Purification and properties of human kidney cortex hexosaminidases A and B. / Wiktorowicz, J. E.; Awasthi, Y. C.; Kurosky, A.; Srivastava, Satish.

In: Biochemical Journal, Vol. 165, No. 1, 1977, p. 49-53.

Research output: Contribution to journalArticle

Wiktorowicz, JE, Awasthi, YC, Kurosky, A & Srivastava, S 1977, 'Purification and properties of human kidney cortex hexosaminidases A and B', Biochemical Journal, vol. 165, no. 1, pp. 49-53.
Wiktorowicz JE, Awasthi YC, Kurosky A, Srivastava S. Purification and properties of human kidney cortex hexosaminidases A and B. Biochemical Journal. 1977;165(1):49-53.
Wiktorowicz, J. E. ; Awasthi, Y. C. ; Kurosky, A. ; Srivastava, Satish. / Purification and properties of human kidney cortex hexosaminidases A and B. In: Biochemical Journal. 1977 ; Vol. 165, No. 1. pp. 49-53.
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