Purification, crystallization and initial X-ray analysis of the head-tail connector of bacteriophage φ29

M. O. Badasso, Petr Leiman, Y. Tao, Y. He, D. H. Ohlendorf, M. G. Rossmann, D. Anderson

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The head-tail connector of bacteriophage φ29, an oligomer of gene product 10 (gp10), was crystallized into various forms. The most useful of these were an orthorhombic P22121 form (unit-cell parameters a = 143.0, b = 157.0, c = 245.2 Å), a monoclinic C2 form (a = 160.7, b = 143.6, c = 221.0 Å, β = 97.8°) and another monoclinic C2 form (a = 177.0, b = 169.1, c = 185.2 Å, β = 114.1°). Frozen crystals diffracted to about 3.2 Å resolution. There is one connector per crystallographic asymmetric unit in each case. Rotation functions show the connector to be a dodecamer. Translation functions readily determined the position of the 12-fold axis in each unit cell. The structure is being determined by 12-fold electron-density averaging within each crystal and by averaging between the various crystal forms.

Original languageEnglish (US)
Pages (from-to)1187-1190
Number of pages4
JournalActa Crystallographica Section D: Biological Crystallography
Volume56
Issue number9
DOIs
StatePublished - 2000
Externally publishedYes

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bacteriophages
Bacteriophages
connectors
X ray analysis
Crystallization
purification
Purification
Head
X-Rays
crystallization
Crystals
crystals
x rays
Electrons
cells
oligomers
Oligomers
genes
Carrier concentration
Genes

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biophysics
  • Condensed Matter Physics
  • Structural Biology

Cite this

Purification, crystallization and initial X-ray analysis of the head-tail connector of bacteriophage φ29. / Badasso, M. O.; Leiman, Petr; Tao, Y.; He, Y.; Ohlendorf, D. H.; Rossmann, M. G.; Anderson, D.

In: Acta Crystallographica Section D: Biological Crystallography, Vol. 56, No. 9, 2000, p. 1187-1190.

Research output: Contribution to journalArticle

Badasso, M. O. ; Leiman, Petr ; Tao, Y. ; He, Y. ; Ohlendorf, D. H. ; Rossmann, M. G. ; Anderson, D. / Purification, crystallization and initial X-ray analysis of the head-tail connector of bacteriophage φ29. In: Acta Crystallographica Section D: Biological Crystallography. 2000 ; Vol. 56, No. 9. pp. 1187-1190.
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