Purification, crystallization and X-ray diffraction analysis of the C-terminal protease domain of Venezuelan equine encephalitis virus nsP2

Andrew T. Russo; Stanley J. Watowich

doi:10.1107/S1744309106014667

Purification, crystallization and X-ray diffraction analysis of the C-terminal protease domain of Venezuelan equine encephalitis virus nsP2

Andrew T. Russo
, Stanley J. Watowich

Biochemistry & Molecular Biology

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

The C-terminal region of Venezuelan equine encephalitis virus (VEEV) nsP2 is responsible for proteolytic processing of the VEEV polyprotein replication complex. This action regulates the activity of the replication complex and is essential for viral replication, thus making nsP2 a very attractive target for development of VEEV therapeutics. The 338-amino-acid C-terminal region of VEEV nsP2 has been overexpressed in Escherichia coli, purified and crystallized. Crystals diffract to beyond 2.5 Å resolution and belong to the orthorhombic space group P2₁2₁2₁. Isomorphous heavy-atom derivatives suitable for phase analysis have been obtained and work on building a complete structural model is under way.

Original language	English (US)
Pages (from-to)	514-517
Number of pages	4
Journal	Acta Crystallographica Section F: Structural Biology and Crystallization Communications
Volume	62
Issue number	6
DOIs	https://doi.org/10.1107/S1744309106014667
State	Published - Jun 2006

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Genetics
Condensed Matter Physics

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Purification, crystallization and X-ray diffraction analysis of the C-terminal protease domain of Venezuelan equine encephalitis virus nsP2. / Russo, Andrew T.; Watowich, Stanley J.
In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications, Vol. 62, No. 6, 06.2006, p. 514-517.

Research output: Contribution to journal › Article › peer-review

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Purification, crystallization and X-ray diffraction analysis of the C-terminal protease domain of Venezuelan equine encephalitis virus nsP2

Abstract

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