Purification of fatty acid ethyl esters by solid-phase extraction and high-performance liquid chromatography

Thomas G. Bernhardt, Paul A. Cannistraro, David A. Bird, Kathleen M. Doyle, Michael Laposata

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

We have developed a two-step method to purify fatty acid ethyl esters (FAEE) using solid-phase extraction (SPE), with a recovery of 70±3% (mean±S.E.M.) as assessed using ethyl oleate as a recovery marker from a standard lipid mixture in hexane. The first step of the SPE procedure involves application of a lipid mixture to an aminopropyl-silica column with simultaneous elution of FAEE and cholesteryl esters from the column with hexane. Gas chromatographic analysis of FAEE without interference from cholesteryl esters may be performed using the eluate from the aminopropyl-silica column, thus eliminating the need for an octadecylsilyl (ODS) column in this case. The FAEE can then be separated from the cholesteryl esters, if necessary, by chromatography on an ODS column and elution with isopropanol-water (5:1, v/v). Both the aminopropyl-silica and ODS columns were found to be effective for up to four uses. To permit isolation of specific FAEE species following isolation of total FAEE by the two-step SPE method, we have also developed a purification scheme for individual FAEE by high-performance liquid chromatography (HPLC). Thus, this simple method allows for reproducible isolation of total FAEE by SPE and isolation of individual FAEE species by HPLC.

Original languageEnglish (US)
Pages (from-to)189-196
Number of pages8
JournalJournal of Chromatography B: Biomedical Applications
Volume675
Issue number2
DOIs
StatePublished - Jan 26 1996
Externally publishedYes

Fingerprint

High performance liquid chromatography
Purification
Esters
Fatty Acids
Cholesterol Esters
Hexanes
Silicon Dioxide
Lipids
Recovery
2-Propanol
Chromatography
Gas chromatography
Water

Keywords

  • Fatty acid ethyl esters
  • Solid-phase extraction

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Purification of fatty acid ethyl esters by solid-phase extraction and high-performance liquid chromatography. / Bernhardt, Thomas G.; Cannistraro, Paul A.; Bird, David A.; Doyle, Kathleen M.; Laposata, Michael.

In: Journal of Chromatography B: Biomedical Applications, Vol. 675, No. 2, 26.01.1996, p. 189-196.

Research output: Contribution to journalArticle

Bernhardt, Thomas G. ; Cannistraro, Paul A. ; Bird, David A. ; Doyle, Kathleen M. ; Laposata, Michael. / Purification of fatty acid ethyl esters by solid-phase extraction and high-performance liquid chromatography. In: Journal of Chromatography B: Biomedical Applications. 1996 ; Vol. 675, No. 2. pp. 189-196.
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