PURIFICATION OF HUMAN PLATELET MONOAMINE OXIDASE B BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY.

Ghulam Ansari, Nutan T. Patel, Richard R. Fritz, Creed W. Abell

Research output: Contribution to journalArticle

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Abstract

Human platelet monoamine oxidase (MAO B), a membrane bound enzyme was purified to homogeneity by DEAE-Sephacel column chromatography, chromatofocusing, and high performance liquid chromatography (HPLC). The crucial purification step was HPLC on a anion exchange column (SynChropak AX 300). The HPLC column was eluted initially with potassium phosphate buffer (100 mM, pH 7. 4) for 10 min at a flow rate of 1. 0 ml/min, followed by a gradient (0-1%) of octyl- beta -D-glucopyranoside (octylglucoside) in the same buffer for 10 min, and finally with buffered octylglucoside (1%) for 40 min. The extent of homogeneity and the subunit M//r (approximately 59,000) of MAO B were determined by sodium-dodecyl sulfate polyacrylamide gel electrophoresis followed by silver staining for proteins.

Original languageEnglish (US)
Pages (from-to)1407-1419
Number of pages13
JournalJournal of Liquid Chromatography
Volume6
Issue number8
StatePublished - 1983

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Monoamine Oxidase
High performance liquid chromatography
Platelets
Purification
Blood Platelets
High Pressure Liquid Chromatography
Buffers
Silver Proteins
Column chromatography
Silver Staining
Distillation columns
Electrophoresis
Sodium Dodecyl Sulfate
Anions
Chromatography
Polyacrylamide Gel Electrophoresis
Ion exchange
Flow rate
Membranes
Enzymes

ASJC Scopus subject areas

  • Molecular Medicine

Cite this

PURIFICATION OF HUMAN PLATELET MONOAMINE OXIDASE B BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY. / Ansari, Ghulam; Patel, Nutan T.; Fritz, Richard R.; Abell, Creed W.

In: Journal of Liquid Chromatography, Vol. 6, No. 8, 1983, p. 1407-1419.

Research output: Contribution to journalArticle

Ansari, Ghulam ; Patel, Nutan T. ; Fritz, Richard R. ; Abell, Creed W. / PURIFICATION OF HUMAN PLATELET MONOAMINE OXIDASE B BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY. In: Journal of Liquid Chromatography. 1983 ; Vol. 6, No. 8. pp. 1407-1419.
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