Purification of the structural and nonstructural proteins of St. Louis encephalitis virus

A. Vance Vorndam, Dennis W. Trent

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

We have developed a procedure for purifying both the structural and nonstructural proteins of flaviviruses from lysates of infected cell cultures. The procedure involves: (l)immunoprecipitation to concentrate viral proteins and eliminate most of the cellular proteins, (2) preparative polyacrylamide gel electrophoresis to separate the viral proteins, and (3) hydroxyapatite chromatography, which eliminates most of the unlabeled cellular protein. This procedure offers an improvement over previous purification schemes in that there is no loss of viral proteins after the immunoprecipitation step, any combination of labeling isotopes may be used, and it is not necessary to soak proteins out of gel slices.

Original languageEnglish (US)
Pages (from-to)283-291
Number of pages9
JournalJournal of Virological Methods
Volume9
Issue number4
DOIs
StatePublished - 1984

Fingerprint

St. Louis Encephalitis Viruses
Viral Proteins
Immunoprecipitation
Proteins
Isotope Labeling
Flavivirus
Durapatite
Chromatography
Polyacrylamide Gel Electrophoresis
Cell Culture Techniques
Gels

Keywords

  • flavivirus
  • protein purification

ASJC Scopus subject areas

  • Virology

Cite this

Purification of the structural and nonstructural proteins of St. Louis encephalitis virus. / Vorndam, A. Vance; Trent, Dennis W.

In: Journal of Virological Methods, Vol. 9, No. 4, 1984, p. 283-291.

Research output: Contribution to journalArticle

Vorndam, A. Vance ; Trent, Dennis W. / Purification of the structural and nonstructural proteins of St. Louis encephalitis virus. In: Journal of Virological Methods. 1984 ; Vol. 9, No. 4. pp. 283-291.
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