Purified choleragenoid does not induce migrating action potential complex activity in rabbit lleum in vivo

C. D. Lind, R. L. Guerrant, A. Kurosky, J. R. Mathias

    Research output: Contribution to journalArticle

    Abstract

    The migrating action potential complex (MAPC), a single ring contraction that propels luminal contents aborad, is elicted by Vibrio cholerae and its enterotoxin, choleragen (A1A2B5), in rabbit ileal loops in vivo. Choleragenoid (B5; the binding subunit) was shown previously to induce MAPCs without activating the adenylate cyclase system and without stimulating fluid output. We restudied purified B5 (extracted by high-performance liquid chromatography) to assess its effects on the myoelectric activity of rabbit ileum: can MAPC activity can be induced without associated fluid production? Four different B5 preparations and two controls, V. cholerae and sterile saline, were used. MAPC activity and ileal fluid output were significantly increased in animals inoculated with A1A2B5 (V. cholerae-El Tor) compared with all other preparations and controls. Importantly, MAPC activity and fluid output in all B5 groups did not differ from those in saline controls, demonstrating that purified B5 does not induce MAPC activity in rabbit ilium in vivo. The previous study showing B5-induced MAPCs may have resulted from A1A2B5 contamination. These results suggest A1A2B5-induced adenylate cyclase activation; myoelectric activity may be interrelated with holotoxin binding that causes a neural response.

    Original languageEnglish (US)
    Pages (from-to)647-651
    Number of pages5
    JournalJournal of Pharmacology and Experimental Therapeutics
    Volume258
    Issue number2
    StatePublished - 1991

    Fingerprint

    Cholera Toxin
    Action Potentials
    Vibrio cholerae
    Rabbits
    Adenylyl Cyclases
    Ilium
    Ileum
    High Pressure Liquid Chromatography

    ASJC Scopus subject areas

    • Pharmacology

    Cite this

    Purified choleragenoid does not induce migrating action potential complex activity in rabbit lleum in vivo. / Lind, C. D.; Guerrant, R. L.; Kurosky, A.; Mathias, J. R.

    In: Journal of Pharmacology and Experimental Therapeutics, Vol. 258, No. 2, 1991, p. 647-651.

    Research output: Contribution to journalArticle

    Lind, C. D. ; Guerrant, R. L. ; Kurosky, A. ; Mathias, J. R. / Purified choleragenoid does not induce migrating action potential complex activity in rabbit lleum in vivo. In: Journal of Pharmacology and Experimental Therapeutics. 1991 ; Vol. 258, No. 2. pp. 647-651.
    @article{444625de3855498b899155e42bba2ba3,
    title = "Purified choleragenoid does not induce migrating action potential complex activity in rabbit lleum in vivo",
    abstract = "The migrating action potential complex (MAPC), a single ring contraction that propels luminal contents aborad, is elicted by Vibrio cholerae and its enterotoxin, choleragen (A1A2B5), in rabbit ileal loops in vivo. Choleragenoid (B5; the binding subunit) was shown previously to induce MAPCs without activating the adenylate cyclase system and without stimulating fluid output. We restudied purified B5 (extracted by high-performance liquid chromatography) to assess its effects on the myoelectric activity of rabbit ileum: can MAPC activity can be induced without associated fluid production? Four different B5 preparations and two controls, V. cholerae and sterile saline, were used. MAPC activity and ileal fluid output were significantly increased in animals inoculated with A1A2B5 (V. cholerae-El Tor) compared with all other preparations and controls. Importantly, MAPC activity and fluid output in all B5 groups did not differ from those in saline controls, demonstrating that purified B5 does not induce MAPC activity in rabbit ilium in vivo. The previous study showing B5-induced MAPCs may have resulted from A1A2B5 contamination. These results suggest A1A2B5-induced adenylate cyclase activation; myoelectric activity may be interrelated with holotoxin binding that causes a neural response.",
    author = "Lind, {C. D.} and Guerrant, {R. L.} and A. Kurosky and Mathias, {J. R.}",
    year = "1991",
    language = "English (US)",
    volume = "258",
    pages = "647--651",
    journal = "Journal of Pharmacology and Experimental Therapeutics",
    issn = "0022-3565",
    publisher = "American Society for Pharmacology and Experimental Therapeutics",
    number = "2",

    }

    TY - JOUR

    T1 - Purified choleragenoid does not induce migrating action potential complex activity in rabbit lleum in vivo

    AU - Lind, C. D.

    AU - Guerrant, R. L.

    AU - Kurosky, A.

    AU - Mathias, J. R.

    PY - 1991

    Y1 - 1991

    N2 - The migrating action potential complex (MAPC), a single ring contraction that propels luminal contents aborad, is elicted by Vibrio cholerae and its enterotoxin, choleragen (A1A2B5), in rabbit ileal loops in vivo. Choleragenoid (B5; the binding subunit) was shown previously to induce MAPCs without activating the adenylate cyclase system and without stimulating fluid output. We restudied purified B5 (extracted by high-performance liquid chromatography) to assess its effects on the myoelectric activity of rabbit ileum: can MAPC activity can be induced without associated fluid production? Four different B5 preparations and two controls, V. cholerae and sterile saline, were used. MAPC activity and ileal fluid output were significantly increased in animals inoculated with A1A2B5 (V. cholerae-El Tor) compared with all other preparations and controls. Importantly, MAPC activity and fluid output in all B5 groups did not differ from those in saline controls, demonstrating that purified B5 does not induce MAPC activity in rabbit ilium in vivo. The previous study showing B5-induced MAPCs may have resulted from A1A2B5 contamination. These results suggest A1A2B5-induced adenylate cyclase activation; myoelectric activity may be interrelated with holotoxin binding that causes a neural response.

    AB - The migrating action potential complex (MAPC), a single ring contraction that propels luminal contents aborad, is elicted by Vibrio cholerae and its enterotoxin, choleragen (A1A2B5), in rabbit ileal loops in vivo. Choleragenoid (B5; the binding subunit) was shown previously to induce MAPCs without activating the adenylate cyclase system and without stimulating fluid output. We restudied purified B5 (extracted by high-performance liquid chromatography) to assess its effects on the myoelectric activity of rabbit ileum: can MAPC activity can be induced without associated fluid production? Four different B5 preparations and two controls, V. cholerae and sterile saline, were used. MAPC activity and ileal fluid output were significantly increased in animals inoculated with A1A2B5 (V. cholerae-El Tor) compared with all other preparations and controls. Importantly, MAPC activity and fluid output in all B5 groups did not differ from those in saline controls, demonstrating that purified B5 does not induce MAPC activity in rabbit ilium in vivo. The previous study showing B5-induced MAPCs may have resulted from A1A2B5 contamination. These results suggest A1A2B5-induced adenylate cyclase activation; myoelectric activity may be interrelated with holotoxin binding that causes a neural response.

    UR - http://www.scopus.com/inward/record.url?scp=0026091152&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=0026091152&partnerID=8YFLogxK

    M3 - Article

    VL - 258

    SP - 647

    EP - 651

    JO - Journal of Pharmacology and Experimental Therapeutics

    JF - Journal of Pharmacology and Experimental Therapeutics

    SN - 0022-3565

    IS - 2

    ER -