Purine excretion by mammalian cells deficient in adenosine kinase

T. S. Chan, K. Ishii, C. Long, H. Green

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

An adenosine kinaseless (AK-) mutant of the mouse fibroblast line 3T6 was obtained in a cell culture by the evolution of resistance to 6 thiomethylpurine ribonucleoside and tubercidin. The mutant excretes purines (xanthine and hypoxanthine) into the culture medium. Human or mouse cells lacking hypoxanthine guanine phosphoribosyl transferase (HPT-) excrete increased amounts of purines, but a human cell mutant lacking both HPT and AK excretes considerably more hypoxanthine. The difference in hypoxanthine excretion between the HPT- mutant and the HPT- AK- mutant originates from the adenosine normally reutilized through the activity of adenosine kinase. The activity of adenosine kinase is essential to retard the adenosine cycle and to prevent cellular loss of purines.

Original languageEnglish (US)
Pages (from-to)315-321
Number of pages7
JournalJournal of Cellular Physiology
Volume81
Issue number3
StatePublished - 1973
Externally publishedYes

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Adenosine Kinase
Hypoxanthine
Cells
Adenosine
Purines
Guanine
Transferases
Tubercidin
Ribonucleosides
Xanthine
Fibroblasts
Cell culture
Culture Media
purine
Cell Culture Techniques

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Physiology

Cite this

Chan, T. S., Ishii, K., Long, C., & Green, H. (1973). Purine excretion by mammalian cells deficient in adenosine kinase. Journal of Cellular Physiology, 81(3), 315-321.

Purine excretion by mammalian cells deficient in adenosine kinase. / Chan, T. S.; Ishii, K.; Long, C.; Green, H.

In: Journal of Cellular Physiology, Vol. 81, No. 3, 1973, p. 315-321.

Research output: Contribution to journalArticle

Chan, TS, Ishii, K, Long, C & Green, H 1973, 'Purine excretion by mammalian cells deficient in adenosine kinase', Journal of Cellular Physiology, vol. 81, no. 3, pp. 315-321.
Chan, T. S. ; Ishii, K. ; Long, C. ; Green, H. / Purine excretion by mammalian cells deficient in adenosine kinase. In: Journal of Cellular Physiology. 1973 ; Vol. 81, No. 3. pp. 315-321.
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