Pyruvate in organ transplantation

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Pyruvate (PY) is a 3-carbon compound present in human tissues and physiologically used by cells as an energetic substrate in anaerobic conditions. In the last few years, we have successfully used PY to protect small bowel (SB), liver, and kidneys from ischemia/reperfusion injury in several experimental models. Although the mechanism of protection is not fully clarified, we have shown increased tissue levels of adenosine driphosphate (ATP) during ischemia. This suggests that providing supra-physiologic concentrations of PY during anaerobic glycolysis might enable the cells to remain viable during prolonged hypoxia. Furthermore, mechanisms such as direct inhibition of oxygen flee radical formation, abrogation of neutrophilic infiltration and reduced up-regulation of adhesion molecules have also been documented in these studies. In light of these findings, we evaluated the efficacy of PY in organ preservation and transplantation. We demonstrated a protective effect on intestinal preservation injury and during acute rejection. Oral PY treatment induced immunologic changes in rejecting allograft, inhibiting perforin and granzyme-b expression and leukocytic infiltration. Protection was also documented on livers after prolonged hypothermic preservation using a PY based preservation solution. Additionally, isolated pancreatic islets were cultured in PY enriched media and maintained viable for up to 120 days followed by in vitro testing and transplantation revealing a well preserved function. All these findings suggest that PY is a potentially beneficial nutrient in patients undergoing organ transplantation and that future clinical application of PY in this field should be encouraged.

Original languageEnglish (US)
Pages (from-to)216-218
Number of pages3
JournalJournal of Parenteral and Enteral Nutrition
Volume25
Issue number4
StatePublished - 2001
Externally publishedYes

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organ transplantation
Organ Transplantation
ischemia
Pyruvic Acid
liver
allografting
islets of Langerhans
glycolysis
adenosine
anaerobic conditions
protective effect
adhesion
hypoxia
mouth
kidneys
cells
oxygen
carbon
nutrients
testing

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Food Science

Cite this

Pyruvate in organ transplantation. / Cicalese, Luca.

In: Journal of Parenteral and Enteral Nutrition, Vol. 25, No. 4, 2001, p. 216-218.

Research output: Contribution to journalArticle

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