QF-SSOP: Real-time optical property corrected fluorescence imaging

Pablo A. Valdes, Joseph P. Angelo, Hak Soo Choi, Sylvain Gioux

Research output: Contribution to journalArticlepeer-review

40 Scopus citations


Fluorescence imaging is well suited to provide image guidance during resections in oncologic and vascular surgery. However, the distorting effects of tissue optical properties on the emitted fluorescence are poorly compensated for on even the most advanced fluorescence image guidance systems, leading to subjective and inaccurate estimates of tissue fluorophore concentrations. Here we present a novel fluorescence imaging technique that performs real-time (i.e., video rate) optical property corrected fluorescence imaging. We perform full field of view simultaneous imaging of tissue optical properties using Single Snapshot of Optical Properties (SSOP) and fluorescence detection. The estimated optical properties are used to correct the emitted fluorescence with a quantitative fluorescence model to provide quantitative fluorescence-Single Snapshot of Optical Properties (qF-SSOP) images with less than 5% error. The technique is rigorous, fast, and quantitative, enabling ease of integration into the surgical workflow with the potential to improve molecular guidance intraoperatively.

Original languageEnglish (US)
Article number#295940
Pages (from-to)3597-3605
Number of pages9
JournalBiomedical Optics Express
Issue number8
StatePublished - Aug 1 2017
Externally publishedYes


  • Image analysis
  • Medical and biological imaging

ASJC Scopus subject areas

  • Biotechnology
  • Atomic and Molecular Physics, and Optics


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