Quantification of ceramide levels in mammalian cells by high performance liquid chromatography coupled to tandem mass spectrometry with multiple-reaction-monitoring mode (HPLC-MS/MS-MRM)

Kangling Zhang; Teka Ann S. Haynes; Maria Filippova; Valery Filippov; Penelope J. Duerksen-Hughes

doi:10.1039/c1ay05022b

Quantification of ceramide levels in mammalian cells by high performance liquid chromatography coupled to tandem mass spectrometry with multiple-reaction-monitoring mode (HPLC-MS/MS-MRM)

Kangling Zhang, Teka Ann S. Haynes, Maria Filippova, Valery Filippov, Penelope J. Duerksen-Hughes

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Ceramides play an important role in a variety of cellular functions including cell differentiation and apoptosis, responses to DNA damage and stress, and transcriptional events. Detection of ceramides in mammalian cells is required for many biological studies. Here, we report a validated method using LC-MS/MS-MRM on an Agilent 6410 triple quadrupole mass spectrometer to simultaneously quantify six ceramides extracted from mammalian cell lysates following a 5 min HPLC gradient. This method demonstrated outstanding sensitivity, accuracy, reproducibility, and speed of analysis.

Original language	English (US)
Pages (from-to)	1193-1197
Number of pages	5
Journal	Analytical Methods
Volume	3
Issue number	5
DOIs	https://doi.org/10.1039/c1ay05022b
State	Published - May 2011
Externally published	Yes

ASJC Scopus subject areas

Analytical Chemistry
General Chemical Engineering
General Engineering

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Quantification of ceramide levels in mammalian cells by high performance liquid chromatography coupled to tandem mass spectrometry with multiple-reaction-monitoring mode (HPLC-MS/MS-MRM). / Zhang, Kangling; Haynes, Teka Ann S.; Filippova, Maria et al.
In: Analytical Methods, Vol. 3, No. 5, 05.2011, p. 1193-1197.

Research output: Contribution to journal › Article › peer-review

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abstract = "Ceramides play an important role in a variety of cellular functions including cell differentiation and apoptosis, responses to DNA damage and stress, and transcriptional events. Detection of ceramides in mammalian cells is required for many biological studies. Here, we report a validated method using LC-MS/MS-MRM on an Agilent 6410 triple quadrupole mass spectrometer to simultaneously quantify six ceramides extracted from mammalian cell lysates following a 5 min HPLC gradient. This method demonstrated outstanding sensitivity, accuracy, reproducibility, and speed of analysis.",

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AU - Filippov, Valery

AU - Duerksen-Hughes, Penelope J.

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AB - Ceramides play an important role in a variety of cellular functions including cell differentiation and apoptosis, responses to DNA damage and stress, and transcriptional events. Detection of ceramides in mammalian cells is required for many biological studies. Here, we report a validated method using LC-MS/MS-MRM on an Agilent 6410 triple quadrupole mass spectrometer to simultaneously quantify six ceramides extracted from mammalian cell lysates following a 5 min HPLC gradient. This method demonstrated outstanding sensitivity, accuracy, reproducibility, and speed of analysis.

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Quantification of ceramide levels in mammalian cells by high performance liquid chromatography coupled to tandem mass spectrometry with multiple-reaction-monitoring mode (HPLC-MS/MS-MRM)

Abstract

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