Quantification of ceramide levels in mammalian cells by high performance liquid chromatography coupled to tandem mass spectrometry with multiple-reaction-monitoring mode (HPLC-MS/MS-MRM)

Kangling Zhang, Teka Ann S Haynes, Maria Filippova, Valery Filippov, Penelope J. Duerksen-Hughes

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Ceramides play an important role in a variety of cellular functions including cell differentiation and apoptosis, responses to DNA damage and stress, and transcriptional events. Detection of ceramides in mammalian cells is required for many biological studies. Here, we report a validated method using LC-MS/MS-MRM on an Agilent 6410 triple quadrupole mass spectrometer to simultaneously quantify six ceramides extracted from mammalian cell lysates following a 5 min HPLC gradient. This method demonstrated outstanding sensitivity, accuracy, reproducibility, and speed of analysis.

Original languageEnglish (US)
Pages (from-to)1193-1197
Number of pages5
JournalAnalytical Methods
Volume3
Issue number5
DOIs
StatePublished - May 2011
Externally publishedYes

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Ceramides
High performance liquid chromatography
Mass spectrometry
Cells
Monitoring
Mass spectrometers
Cell death
DNA
Apoptosis

ASJC Scopus subject areas

  • Analytical Chemistry
  • Engineering(all)
  • Chemical Engineering(all)

Cite this

Quantification of ceramide levels in mammalian cells by high performance liquid chromatography coupled to tandem mass spectrometry with multiple-reaction-monitoring mode (HPLC-MS/MS-MRM). / Zhang, Kangling; Haynes, Teka Ann S; Filippova, Maria; Filippov, Valery; Duerksen-Hughes, Penelope J.

In: Analytical Methods, Vol. 3, No. 5, 05.2011, p. 1193-1197.

Research output: Contribution to journalArticle

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