Quantification of PARP activity in human tissues: Ex Vivo assays in blood cells and immunohistochemistry in human biopsies

Eszter M. Horvath, Zsuzsanna K. Zsengellér, Csaba Szabo

    Research output: Chapter in Book/Report/Conference proceedingChapter

    5 Scopus citations

    Abstract

    Poly (ADP-ribosyl)ation of proteins is a posttranslational modification mediated by poly (ADP-ribose) polymerases (PARPs) that uses NAD + as substrate to form the negatively charged polymer of poly (ADP-ribose) (PAR). After DNA damage, PARP-1 is responsible for approximately 90% of the total cellular PARylation activity. Numerous studies showed activation of PARP-1 in various conditions associated with oxidative and nitrosative stress, such as ischemia-reperfusion injury, diabetes mellitus, and inflammation, and also proved the beneficial effects of PARP inhibitors. Pharmacological inhibitors of PARP move toward clinical testing for a variety of indications, including cardioprotection and malignant tumors. Some of the compounds are already in clinical trials. These advances necessitate the detection of PARP activation in human tissues. In the present chapter, we review specific methods used to detect PARP activation in human circulating leukocytes and human tissue sections.

    Original languageEnglish (US)
    Title of host publicationPoly(ADP-ribose) Polymerase
    Subtitle of host publicationMethods and Protocols
    EditorsAlexei V. Tulin
    Pages267-275
    Number of pages9
    DOIs
    StatePublished - Oct 24 2011

    Publication series

    NameMethods in Molecular Biology
    Volume780
    ISSN (Print)1064-3745

    Keywords

    • Biopsy
    • Circulating leukocytes
    • DAB
    • Immunohistochemistry
    • PARP
    • Poly(ADP-ribose)

    ASJC Scopus subject areas

    • Molecular Biology
    • Genetics

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  • Cite this

    Horvath, E. M., Zsengellér, Z. K., & Szabo, C. (2011). Quantification of PARP activity in human tissues: Ex Vivo assays in blood cells and immunohistochemistry in human biopsies. In A. V. Tulin (Ed.), Poly(ADP-ribose) Polymerase: Methods and Protocols (pp. 267-275). (Methods in Molecular Biology; Vol. 780). https://doi.org/10.1007/978-1-61779-270-0_16