Quantification of PARP activity in human tissues

Ex vivo assays in blood cells and immunohistochemistry in human biopsies

Eszter M. Horvath, Zsuzsanna K. Zsengellér, Csaba Szabo

    Research output: Chapter in Book/Report/Conference proceedingChapter

    1 Citation (Scopus)

    Abstract

    Poly(ADP-ribosyl)ation of proteins is a posttranslational modification mediated by poly(ADP-ribose) polymerases (PARPs) that use NAD+ as substrate to form the negatively charged polymer of poly(ADP-ribose) (PAR). After DNA damage, PARP-1 is responsible for approximately 90% of the total cellular PARylation activity. Numerous studies showed activation of PARP-1 in various conditions associated with oxidative and nitrosative stress, such as ischemia-reperfusion injury, diabetes mellitus, and inflammation, and also proved the beneficial effects of PARP inhibitors. Several pharmacological inhibitors of PARP moved toward clinical testing for a variety of indications, including cardioprotection and malignant tumors, and in late 2014, olaparib became the first PARP inhibitor approved for human use for the therapy of ovarian cancer. These advances necessitate the detection of PARP activation in human tissues. In the present chapter, we review specific methods used to detect PARP activation in human circulating leukocytes and in human tissue sections.

    Original languageEnglish (US)
    Title of host publicationMethods in Molecular Biology
    PublisherHumana Press Inc.
    Pages19-26
    Number of pages8
    Volume1608
    DOIs
    StatePublished - 2017

    Publication series

    NameMethods in Molecular Biology
    Volume1608
    ISSN (Print)1064-3745

    Fingerprint

    Poly(ADP-ribose) Polymerases
    Human Activities
    Blood Cells
    Immunohistochemistry
    Biopsy
    Poly Adenosine Diphosphate Ribose
    Post Translational Protein Processing
    Reperfusion Injury
    NAD
    Ovarian Neoplasms
    DNA Damage
    Diabetes Mellitus
    Polymers
    Oxidative Stress
    Leukocytes
    Pharmacology
    Inflammation
    Poly(ADP-ribose) Polymerase Inhibitors
    Neoplasms
    Poly (ADP-Ribose) Polymerase-1

    Keywords

    • Biopsy
    • Circulating leukocytes
    • DAB
    • Immunohistochemistry
    • PARP
    • Poly(ADP-ribose)

    ASJC Scopus subject areas

    • Molecular Biology
    • Genetics

    Cite this

    Horvath, E. M., Zsengellér, Z. K., & Szabo, C. (2017). Quantification of PARP activity in human tissues: Ex vivo assays in blood cells and immunohistochemistry in human biopsies. In Methods in Molecular Biology (Vol. 1608, pp. 19-26). (Methods in Molecular Biology; Vol. 1608). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-6993-7_2

    Quantification of PARP activity in human tissues : Ex vivo assays in blood cells and immunohistochemistry in human biopsies. / Horvath, Eszter M.; Zsengellér, Zsuzsanna K.; Szabo, Csaba.

    Methods in Molecular Biology. Vol. 1608 Humana Press Inc., 2017. p. 19-26 (Methods in Molecular Biology; Vol. 1608).

    Research output: Chapter in Book/Report/Conference proceedingChapter

    Horvath, EM, Zsengellér, ZK & Szabo, C 2017, Quantification of PARP activity in human tissues: Ex vivo assays in blood cells and immunohistochemistry in human biopsies. in Methods in Molecular Biology. vol. 1608, Methods in Molecular Biology, vol. 1608, Humana Press Inc., pp. 19-26. https://doi.org/10.1007/978-1-4939-6993-7_2
    Horvath EM, Zsengellér ZK, Szabo C. Quantification of PARP activity in human tissues: Ex vivo assays in blood cells and immunohistochemistry in human biopsies. In Methods in Molecular Biology. Vol. 1608. Humana Press Inc. 2017. p. 19-26. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-6993-7_2
    Horvath, Eszter M. ; Zsengellér, Zsuzsanna K. ; Szabo, Csaba. / Quantification of PARP activity in human tissues : Ex vivo assays in blood cells and immunohistochemistry in human biopsies. Methods in Molecular Biology. Vol. 1608 Humana Press Inc., 2017. pp. 19-26 (Methods in Molecular Biology).
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