Quantification of protein metabolism in Vivo for skin, wound, and muscle in severe burn patients

Dennis C. Gore, David L. Chinkes, Steven E. Wolf, Arthur P. Sanford, David N. Herndon, Robert R. Wolfe

Research output: Contribution to journalArticle

27 Scopus citations

Abstract

Background: In response to injury, muscle catabolism can be extensive, and in theory, the wound consumes amino acids to support healing. The purpose of this study is to assess a technique by which in vivo protein kinetics of muscle, wound, and normal skin can be quantified in burn-injured patients. Methods: Study protocol consisting of infusion of d 5 phenylalanine; biopsies of skeletal muscle, skin, and donor-site wound on the leg; quantification of blood flow to total leg, wound, and skin; and sequential blood sampling from the femoral artery and vein. Five-compartment modeling was used to quantify the rates of protein synthesis, breakdown, and phenylalanine transport between muscle, wound, and skin. Results: The study results demonstrated a net release of phenylalanine from muscle yet a net consumption of phenylalanine by the wound. Compared with skin, the wound had a substantially increased rate of protein synthesis and a reduced rate of protein breakdown (p < .01). Transport rates into and out of muscle were significantly higher than those for wound (p < .01). Conclusions: This novel methodology enables in vivo quantification of the integrated response of muscle, wound, and skin protein/amino acid metabolism and confirms the long-held theory of a net catab-olism of muscle and a net anabolism of wound protein in patients after injury. This methodology can be used to assess the metabolic impact of such measures as nutrition, pharma-cologic agents, and surgical procedures. (Journal of Parenteral and Enteral Nutrition 30:331-338, 2006)

Original languageEnglish (US)
Pages (from-to)331-338
Number of pages8
JournalJournal of Parenteral and Enteral Nutrition
Volume30
Issue number4
DOIs
StatePublished - Dec 1 2006

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

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