Quantification of UCP1 function in human brown adipose tissue

Craig Porter

Research output: Contribution to journalReview article

7 Citations (Scopus)

Abstract

Brown adipose tissue (BAT) mitochondria are distinct from their counterparts in other tissues in that ATP production is not their primary physiologic role. BAT mitochondria are equipped with a specialized protein known as uncoupling protein 1 (UCP1). UCP1 short-circuits the electron transport chain, allowing mitochondrial membrane potential to be transduced to heat, making BAT a tissue capable of altering energy expenditure and fuel metabolism in mammals without increasing physical activity. The recent discovery that adult humans have metabolically active BAT has rekindled an interest in this intriguing tissue, with the overarching aim of manipulating BAT function to augment energy expenditure as a countermeasure for obesity and the metabolic abnormalities it incurs. Subsequently, there has been heightened interest in quantifying BAT function and more specifically, determining UCP1-mediated thermogenesis in BAT specimens - including in those obtained from humans. In this article, BAT mitochondrial bioenergetics will be described and compared with more conventional mitochondria in other tissues. The biochemical methods typically used to quantify BAT mitochondrial function will also be discussed in terms of their specificity for assaying UCP1 mediated thermogenesis. Finally, recent data concerning BAT UCP1 function in humans will be described and discussed.

Original languageEnglish (US)
Pages (from-to)167-174
Number of pages8
JournalAdipocyte
Volume6
Issue number2
DOIs
StatePublished - Apr 3 2017

Fingerprint

Brown Adipose Tissue
Energy Metabolism
Mitochondria
Thermogenesis
Uncoupling Protein 1
Mitochondrial Membrane Potential
Electron Transport
Mammals
Obesity
Hot Temperature
Adenosine Triphosphate
Exercise

Keywords

  • Brown fat
  • GDP
  • mitochondria
  • thermogenesis
  • UCP1

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Quantification of UCP1 function in human brown adipose tissue. / Porter, Craig.

In: Adipocyte, Vol. 6, No. 2, 03.04.2017, p. 167-174.

Research output: Contribution to journalReview article

Porter, Craig. / Quantification of UCP1 function in human brown adipose tissue. In: Adipocyte. 2017 ; Vol. 6, No. 2. pp. 167-174.
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