Quantifying the RNA cap epitranscriptome reveals novel caps in cellular and viral RNA

Jin Wang, Bing Liang Alvin Chew, Yong Lai, Hongping Dong, Luang Xu, Seetharamsingh Balamkundu, Weiling Maggie Cai, Liang Cui, Chuan Fa Liu, Xin Yuan Fu, Zhenguo Lin, Pei Yong Shi, Timothy K. Lu, Dahai Luo, Samie R. Jaffrey, Peter C. Dedon

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Chemical modification of transcripts with 5' caps occurs in all organisms. Here, we report a systems-level mass spectrometry-based technique, CapQuant, for quantitative analysis of an organism's cap epitranscriptome. The method was piloted with 21 canonical caps-m7GpppN, m7GpppNm, GpppN, GpppNm, and m2,2,7GpppG-and 5 'metabolite' caps-NAD, FAD, UDP-Glc, UDP-GlcNAc, and dpCoA. Applying CapQuant to RNA from purified dengue virus, Escherichia coli, yeast, mouse tissues, and human cells, we discovered new cap structures in humans and mice (FAD, UDP-Glc, UDP-GlcNAc, and m7Gpppm6A), cell- and tissue-specific variations in cap methylation, and high proportions of caps lacking 2'-O-methylation (m7Gpppm6A in mammals, m7GpppA in dengue virus). While substantial Dimroth-induced loss of m1A and m1Am arose with specific RNA processing conditions, human lymphoblast cells showed no detectable m1A or m1Am in caps. CapQuant accurately captured the preference for purine nucleotides at eukaryotic transcription start sites and the correlation between metabolite levels and metabolite caps.

Original languageEnglish (US)
Pages (from-to)e130
JournalNucleic acids research
Volume47
Issue number20
DOIs
StatePublished - Nov 18 2019

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RNA Caps
Uridine Diphosphate
Viral RNA
Dengue Virus
Flavin-Adenine Dinucleotide
Methylation
RNA
Purine Nucleotides
Transcription Initiation Site
NAD
Mammals
Mass Spectrometry
Yeasts
Escherichia coli

ASJC Scopus subject areas

  • Genetics

Cite this

Wang, J., Alvin Chew, B. L., Lai, Y., Dong, H., Xu, L., Balamkundu, S., ... Dedon, P. C. (2019). Quantifying the RNA cap epitranscriptome reveals novel caps in cellular and viral RNA. Nucleic acids research, 47(20), e130. https://doi.org/10.1093/nar/gkz751

Quantifying the RNA cap epitranscriptome reveals novel caps in cellular and viral RNA. / Wang, Jin; Alvin Chew, Bing Liang; Lai, Yong; Dong, Hongping; Xu, Luang; Balamkundu, Seetharamsingh; Cai, Weiling Maggie; Cui, Liang; Liu, Chuan Fa; Fu, Xin Yuan; Lin, Zhenguo; Shi, Pei Yong; Lu, Timothy K.; Luo, Dahai; Jaffrey, Samie R.; Dedon, Peter C.

In: Nucleic acids research, Vol. 47, No. 20, 18.11.2019, p. e130.

Research output: Contribution to journalArticle

Wang, J, Alvin Chew, BL, Lai, Y, Dong, H, Xu, L, Balamkundu, S, Cai, WM, Cui, L, Liu, CF, Fu, XY, Lin, Z, Shi, PY, Lu, TK, Luo, D, Jaffrey, SR & Dedon, PC 2019, 'Quantifying the RNA cap epitranscriptome reveals novel caps in cellular and viral RNA', Nucleic acids research, vol. 47, no. 20, pp. e130. https://doi.org/10.1093/nar/gkz751
Wang, Jin ; Alvin Chew, Bing Liang ; Lai, Yong ; Dong, Hongping ; Xu, Luang ; Balamkundu, Seetharamsingh ; Cai, Weiling Maggie ; Cui, Liang ; Liu, Chuan Fa ; Fu, Xin Yuan ; Lin, Zhenguo ; Shi, Pei Yong ; Lu, Timothy K. ; Luo, Dahai ; Jaffrey, Samie R. ; Dedon, Peter C. / Quantifying the RNA cap epitranscriptome reveals novel caps in cellular and viral RNA. In: Nucleic acids research. 2019 ; Vol. 47, No. 20. pp. e130.
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