Quantitation of cytokine levels in skin graft donor site wound fluid

L. S. Grayson, J. F. Hansbrough, Ramon Zapata Sirvent, C. A. Dore, J. L. Morgan, M. A. Nicolson

Research output: Contribution to journalArticle

60 Scopus citations


We quantified endogenous levels of multiple cytokines in skin graft donor site wounds in patients with small to moderate-sixed burn injuries. Thirteen patients aged 11 months to 61 years with mean TBSA burn of 4 ± 1 per cent underwent placement of occlusive wound dressings on partial skin thickness donor site wounds. Fluid was aspirated from beneath the dressing on postoperative day 1 and every subsequent 24 h until no further fluid could be obtained. Interleukin-1 alpha (IL-1) and tumour necrosis factor-alpha (TNF-alpha) levels were determined by enzyme-linked immunosorbent assay (ELISA). Epidermal growth factor (EGF), basic-fibroblast growth factor (bFGF), and platelet-derived growth factor (PDGF) were measured by an enzyme immunoassay (EIA). We found substantial levels of EGF and TNF-alpha in the donor site wound fluid in all 13 patients; detectable levels of bFGF in five patients; and elevated levels of IL-1 in three patients. There were no detectable levels of these cytokines in normal human serum. In contrast, there were no measurable levels of PDGF in any patient's wound fluid; the mean level in serum was 1.5 ng/ml ± 0.2 s.e.m. Studies of cytokines in the normal wound healing environment may help in the design of future therapies to augment wound healing.

Original languageEnglish (US)
Pages (from-to)401-405
Number of pages5
Issue number5
StatePublished - Jan 1 1993
Externally publishedYes


ASJC Scopus subject areas

  • Surgery
  • Emergency Medicine
  • Critical Care and Intensive Care Medicine

Cite this

Grayson, L. S., Hansbrough, J. F., Zapata Sirvent, R., Dore, C. A., Morgan, J. L., & Nicolson, M. A. (1993). Quantitation of cytokine levels in skin graft donor site wound fluid. Burns, 19(5), 401-405. https://doi.org/10.1016/0305-4179(93)90061-C