Quantitation of cytokine levels in skin graft donor site wound fluid

L. S. Grayson, J. F. Hansbrough, Ramon Zapata Sirvent, C. A. Dore, J. L. Morgan, M. A. Nicolson

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

We quantified endogenous levels of multiple cytokines in skin graft donor site wounds in patients with small to moderate-sixed burn injuries. Thirteen patients aged 11 months to 61 years with mean TBSA burn of 4 ± 1 per cent underwent placement of occlusive wound dressings on partial skin thickness donor site wounds. Fluid was aspirated from beneath the dressing on postoperative day 1 and every subsequent 24 h until no further fluid could be obtained. Interleukin-1 alpha (IL-1) and tumour necrosis factor-alpha (TNF-alpha) levels were determined by enzyme-linked immunosorbent assay (ELISA). Epidermal growth factor (EGF), basic-fibroblast growth factor (bFGF), and platelet-derived growth factor (PDGF) were measured by an enzyme immunoassay (EIA). We found substantial levels of EGF and TNF-alpha in the donor site wound fluid in all 13 patients; detectable levels of bFGF in five patients; and elevated levels of IL-1 in three patients. There were no detectable levels of these cytokines in normal human serum. In contrast, there were no measurable levels of PDGF in any patient's wound fluid; the mean level in serum was 1.5 ng/ml ± 0.2 s.e.m. Studies of cytokines in the normal wound healing environment may help in the design of future therapies to augment wound healing.

Original languageEnglish (US)
Pages (from-to)401-405
Number of pages5
JournalBurns
Volume19
Issue number5
DOIs
StatePublished - Jan 1 1993
Externally publishedYes

Fingerprint

Tissue Donors
Cytokines
Transplants
Skin
Wounds and Injuries
Interleukin-1alpha
Platelet-Derived Growth Factor
Fibroblast Growth Factor 2
Epidermal Growth Factor
Wound Healing
Tumor Necrosis Factor-alpha
Occlusive Dressings
Bandages
Serum
Immunoenzyme Techniques
Enzyme-Linked Immunosorbent Assay
Therapeutics

ASJC Scopus subject areas

  • Surgery
  • Emergency Medicine
  • Critical Care and Intensive Care Medicine

Cite this

Grayson, L. S., Hansbrough, J. F., Zapata Sirvent, R., Dore, C. A., Morgan, J. L., & Nicolson, M. A. (1993). Quantitation of cytokine levels in skin graft donor site wound fluid. Burns, 19(5), 401-405. https://doi.org/10.1016/0305-4179(93)90061-C

Quantitation of cytokine levels in skin graft donor site wound fluid. / Grayson, L. S.; Hansbrough, J. F.; Zapata Sirvent, Ramon; Dore, C. A.; Morgan, J. L.; Nicolson, M. A.

In: Burns, Vol. 19, No. 5, 01.01.1993, p. 401-405.

Research output: Contribution to journalArticle

Grayson, LS, Hansbrough, JF, Zapata Sirvent, R, Dore, CA, Morgan, JL & Nicolson, MA 1993, 'Quantitation of cytokine levels in skin graft donor site wound fluid', Burns, vol. 19, no. 5, pp. 401-405. https://doi.org/10.1016/0305-4179(93)90061-C
Grayson LS, Hansbrough JF, Zapata Sirvent R, Dore CA, Morgan JL, Nicolson MA. Quantitation of cytokine levels in skin graft donor site wound fluid. Burns. 1993 Jan 1;19(5):401-405. https://doi.org/10.1016/0305-4179(93)90061-C
Grayson, L. S. ; Hansbrough, J. F. ; Zapata Sirvent, Ramon ; Dore, C. A. ; Morgan, J. L. ; Nicolson, M. A. / Quantitation of cytokine levels in skin graft donor site wound fluid. In: Burns. 1993 ; Vol. 19, No. 5. pp. 401-405.
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