Quantitation of reduced glutathione and cysteine in human immunodeficiency virus-infected patients

Elena Sbrana, Adriano Paladini, Emilia Bramanti, Maria C. Spinetti, Giorgio Raspi

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Plasma viral load (VL) values and CD4+ cell count are employed clinically for initiation of therapy in the treatment of patients infected with human immunodeficiency virus (HIV), as previous clinical studies have shown a marked prevalence of acquired immunodeficiency sydrome (AIDS) development in seropositive individuals with VL values over 30 000 copies/mL. Many studies have shown that reduced glutathione (GSH) and cysteine (Cys) deficiency play an important role in the infection. We have developed capillary zone electrophoresis (CZE)-based assays and have used them to investigate the relationship between plasma and intracellular thiol levels and HIV-1 viremia in plasma. Blood samples from healthy volunteers and seropositive patients undergoing different antiretroviral regimes were analyzed in the study. The VL assay was based on CZE-UV detection of viral RNA at 260 nm. Determination of endogenous reduced Cys and GSH was achieved by CZE-UV detection of their mercurial complexes at 200 nm. We found that a decrease in GSH and Cys levels may be associated with disease progress. In fact, reduced GSH and Cys levels appear progressively reduced with increasing VL.

Original languageEnglish (US)
Pages (from-to)1522-1529
Number of pages8
JournalElectrophoresis
Volume25
Issue number10-11
StatePublished - Jun 2004

Fingerprint

Viral Load
Viruses
Glutathione
Cysteine
Capillary Electrophoresis
Electrophoresis
HIV
Plasmas
Assays
Viremia
Viral RNA
CD4 Lymphocyte Count
Sulfhydryl Compounds
HIV-1
Healthy Volunteers
Blood
Therapeutics
Infection

Keywords

  • Capillary electrophoresis
  • Cysteine
  • Glutathione
  • Human immunodeficiency virus
  • Viral load

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Sbrana, E., Paladini, A., Bramanti, E., Spinetti, M. C., & Raspi, G. (2004). Quantitation of reduced glutathione and cysteine in human immunodeficiency virus-infected patients. Electrophoresis, 25(10-11), 1522-1529.

Quantitation of reduced glutathione and cysteine in human immunodeficiency virus-infected patients. / Sbrana, Elena; Paladini, Adriano; Bramanti, Emilia; Spinetti, Maria C.; Raspi, Giorgio.

In: Electrophoresis, Vol. 25, No. 10-11, 06.2004, p. 1522-1529.

Research output: Contribution to journalArticle

Sbrana, E, Paladini, A, Bramanti, E, Spinetti, MC & Raspi, G 2004, 'Quantitation of reduced glutathione and cysteine in human immunodeficiency virus-infected patients', Electrophoresis, vol. 25, no. 10-11, pp. 1522-1529.
Sbrana E, Paladini A, Bramanti E, Spinetti MC, Raspi G. Quantitation of reduced glutathione and cysteine in human immunodeficiency virus-infected patients. Electrophoresis. 2004 Jun;25(10-11):1522-1529.
Sbrana, Elena ; Paladini, Adriano ; Bramanti, Emilia ; Spinetti, Maria C. ; Raspi, Giorgio. / Quantitation of reduced glutathione and cysteine in human immunodeficiency virus-infected patients. In: Electrophoresis. 2004 ; Vol. 25, No. 10-11. pp. 1522-1529.
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