Quantitative and histological assessment of maternal-fetal transmission of Trypanosoma cruzi in guinea pigs: An experimental model of congenital Chagas disease

Jatziri Torres-Vargas, Matilde Jiménez-Coello, Eugenia Guzmán-Marín, Karla Y. Acosta-Viana, Zaida E. Yadon, Eduardo Gutiérrez-Blanco, José Leonardo Guillermo-Cordero, Nisha Garg, Antonio Ortega-Pacheco

    Research output: Contribution to journalArticle

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    Abstract

    Objective: We evaluated the effect of Trypanosoma cruzi infection on fertility, gestation outcome, and maternal-fetal transmission in guinea pigs (Cavia porcellus). Methods: Animals were infected with T. cruzi H4 strain (TcI lineage) before gestation (IBG) or during gestation (IDG). Tissue and sera samples of dams and fetuses were obtained near parturition. Results: All IBG and IDG dams were seropositive by two tests, and exhibited blood parasite load of 1.62±2.2 and 50.1±62 parasites/μl, respectively, by quantitative PCR. Histological evaluation showed muscle fiber degeneration and cellular necrosis in all infected dams. Parasite nests were not detected in infected dams by histology. However, qPCR analysis detected parasites-eq/g heart tissue of 153±104.7 and 169.3±129.4 in IBG and IDG dams, respectively. All fetuses of infected dams were positive for anti-parasite IgG antibodies and tissue parasites by qPCR, but presented a low level of tissue inflammatory infiltrate. Fetuses of IDG (vs. IBG) dams exhibited higher degree of muscle fiber degeneration and cellular necrosis in the heart and skeletal tissues. The placental tissue exhibited no inflammatory lesions and amastigote nests, yet parasites-eq/g of 381.2±34.3 and 79.2±84.9 were detected in IDG and IBG placentas, respectively. Fetal development was compromised, and evidenced by a decline in weight, crow-rump length, and abdominal width in both groups. Conclusions: T. cruzi TcI has a high capacity of congenital transmission even when it was inoculated at a very low dose before or during gestation. Tissue lesions, parasite load, and fetal under development provide evidence for high virulence of the parasite during pregnancy. Despite finding of high parasite burden by qPCR, placentas were protected from cellular damage. Our studies offer an experimental model to study the efficacy of vaccines and drugs against congenital transmission of T. cruzi. These results also call for T. cruzi screening in pregnant women and adequate follow up of the newborns in endemic areas.

    Original languageEnglish (US)
    Article numbere0006222
    JournalPLoS Neglected Tropical Diseases
    Volume12
    Issue number1
    DOIs
    StatePublished - Jan 1 2018

    Fingerprint

    Chagas Disease
    Trypanosoma cruzi
    Guinea Pigs
    Theoretical Models
    Mothers
    Pregnancy
    Parasites
    Parasite Load
    Fetus
    Fetal Development
    Placenta
    Necrosis
    Crows
    Muscles
    Hematologic Tests
    Fertility
    Virulence
    Pregnant Women
    Histology
    Vaccines

    ASJC Scopus subject areas

    • Public Health, Environmental and Occupational Health
    • Infectious Diseases

    Cite this

    Torres-Vargas, J., Jiménez-Coello, M., Guzmán-Marín, E., Acosta-Viana, K. Y., Yadon, Z. E., Gutiérrez-Blanco, E., ... Ortega-Pacheco, A. (2018). Quantitative and histological assessment of maternal-fetal transmission of Trypanosoma cruzi in guinea pigs: An experimental model of congenital Chagas disease. PLoS Neglected Tropical Diseases, 12(1), [e0006222]. https://doi.org/10.1371/journal.pntd.0006222

    Quantitative and histological assessment of maternal-fetal transmission of Trypanosoma cruzi in guinea pigs : An experimental model of congenital Chagas disease. / Torres-Vargas, Jatziri; Jiménez-Coello, Matilde; Guzmán-Marín, Eugenia; Acosta-Viana, Karla Y.; Yadon, Zaida E.; Gutiérrez-Blanco, Eduardo; Guillermo-Cordero, José Leonardo; Garg, Nisha; Ortega-Pacheco, Antonio.

    In: PLoS Neglected Tropical Diseases, Vol. 12, No. 1, e0006222, 01.01.2018.

    Research output: Contribution to journalArticle

    Torres-Vargas, J, Jiménez-Coello, M, Guzmán-Marín, E, Acosta-Viana, KY, Yadon, ZE, Gutiérrez-Blanco, E, Guillermo-Cordero, JL, Garg, N & Ortega-Pacheco, A 2018, 'Quantitative and histological assessment of maternal-fetal transmission of Trypanosoma cruzi in guinea pigs: An experimental model of congenital Chagas disease', PLoS Neglected Tropical Diseases, vol. 12, no. 1, e0006222. https://doi.org/10.1371/journal.pntd.0006222
    Torres-Vargas, Jatziri ; Jiménez-Coello, Matilde ; Guzmán-Marín, Eugenia ; Acosta-Viana, Karla Y. ; Yadon, Zaida E. ; Gutiérrez-Blanco, Eduardo ; Guillermo-Cordero, José Leonardo ; Garg, Nisha ; Ortega-Pacheco, Antonio. / Quantitative and histological assessment of maternal-fetal transmission of Trypanosoma cruzi in guinea pigs : An experimental model of congenital Chagas disease. In: PLoS Neglected Tropical Diseases. 2018 ; Vol. 12, No. 1.
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    abstract = "Objective: We evaluated the effect of Trypanosoma cruzi infection on fertility, gestation outcome, and maternal-fetal transmission in guinea pigs (Cavia porcellus). Methods: Animals were infected with T. cruzi H4 strain (TcI lineage) before gestation (IBG) or during gestation (IDG). Tissue and sera samples of dams and fetuses were obtained near parturition. Results: All IBG and IDG dams were seropositive by two tests, and exhibited blood parasite load of 1.62±2.2 and 50.1±62 parasites/μl, respectively, by quantitative PCR. Histological evaluation showed muscle fiber degeneration and cellular necrosis in all infected dams. Parasite nests were not detected in infected dams by histology. However, qPCR analysis detected parasites-eq/g heart tissue of 153±104.7 and 169.3±129.4 in IBG and IDG dams, respectively. All fetuses of infected dams were positive for anti-parasite IgG antibodies and tissue parasites by qPCR, but presented a low level of tissue inflammatory infiltrate. Fetuses of IDG (vs. IBG) dams exhibited higher degree of muscle fiber degeneration and cellular necrosis in the heart and skeletal tissues. The placental tissue exhibited no inflammatory lesions and amastigote nests, yet parasites-eq/g of 381.2±34.3 and 79.2±84.9 were detected in IDG and IBG placentas, respectively. Fetal development was compromised, and evidenced by a decline in weight, crow-rump length, and abdominal width in both groups. Conclusions: T. cruzi TcI has a high capacity of congenital transmission even when it was inoculated at a very low dose before or during gestation. Tissue lesions, parasite load, and fetal under development provide evidence for high virulence of the parasite during pregnancy. Despite finding of high parasite burden by qPCR, placentas were protected from cellular damage. Our studies offer an experimental model to study the efficacy of vaccines and drugs against congenital transmission of T. cruzi. These results also call for T. cruzi screening in pregnant women and adequate follow up of the newborns in endemic areas.",
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    AU - Guzmán-Marín, Eugenia

    AU - Acosta-Viana, Karla Y.

    AU - Yadon, Zaida E.

    AU - Gutiérrez-Blanco, Eduardo

    AU - Guillermo-Cordero, José Leonardo

    AU - Garg, Nisha

    AU - Ortega-Pacheco, Antonio

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