Quantitative assessment of a circulating depolarizing factor in shock

Brian Button, R. David Baker, Roger A. Vertrees, Shawn Elise Allen, Malcolm S. Brodwick, George Kramer

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Sustained depolarization of cell membranes and cellular edema are known to accompany various forms of circulatory shock and probably contribute to hypovolemia and cellular dysfunction. It has been proposed that a circulating protein is responsible for these effects. In the present study we have confirmed the existence of a circulating depolarizing factor (CDF) in hemorrhagic shock, burn shock, sepsis, and cardiopulmonary bypass. Plasma samples from pigs or sheep in shock were quantitatively assayed for depolarizing activity using a microelectrode method on rat diaphragm in vitro. The depolarizing effect of CDF in vitro was similar in magnitude to that of shock in situ. We conclude that CDF can entirely account for membrane depolarization during shock. The depolarizing effect of CDF was dose-dependent and saturable; it could be reversed by rinsing the diaphragm with Ringer's or control plasma. CDF activity was detectable in plasma within 5 min after a severe scald and gradually increased over the next 25 min. Resuscitation of hemorrhaged pigs, but not burned sheep, eliminated plasma CDF activity.

Original languageEnglish (US)
Pages (from-to)239-244
Number of pages6
JournalShock
Volume15
Issue number3
StatePublished - Mar 2001

Fingerprint

Shock
Diaphragm
Sheep
Swine
Hypovolemia
Hemorrhagic Shock
Microelectrodes
Cardiopulmonary Bypass
Resuscitation
Edema
Sepsis
Cell Membrane
Membranes
Proteins
In Vitro Techniques

Keywords

  • Burns
  • Cardiopulmonary bypass
  • Depolarization
  • Hemorrhage
  • Resuscitation
  • Sepsis
  • Skeletal muscle

ASJC Scopus subject areas

  • Physiology
  • Critical Care and Intensive Care Medicine

Cite this

Button, B., Baker, R. D., Vertrees, R. A., Allen, S. E., Brodwick, M. S., & Kramer, G. (2001). Quantitative assessment of a circulating depolarizing factor in shock. Shock, 15(3), 239-244.

Quantitative assessment of a circulating depolarizing factor in shock. / Button, Brian; Baker, R. David; Vertrees, Roger A.; Allen, Shawn Elise; Brodwick, Malcolm S.; Kramer, George.

In: Shock, Vol. 15, No. 3, 03.2001, p. 239-244.

Research output: Contribution to journalArticle

Button, B, Baker, RD, Vertrees, RA, Allen, SE, Brodwick, MS & Kramer, G 2001, 'Quantitative assessment of a circulating depolarizing factor in shock', Shock, vol. 15, no. 3, pp. 239-244.
Button B, Baker RD, Vertrees RA, Allen SE, Brodwick MS, Kramer G. Quantitative assessment of a circulating depolarizing factor in shock. Shock. 2001 Mar;15(3):239-244.
Button, Brian ; Baker, R. David ; Vertrees, Roger A. ; Allen, Shawn Elise ; Brodwick, Malcolm S. ; Kramer, George. / Quantitative assessment of a circulating depolarizing factor in shock. In: Shock. 2001 ; Vol. 15, No. 3. pp. 239-244.
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