Quantitative determination of pravastatin and its metabolite 3α-hydroxy pravastatin in plasma and urine of pregnant patients by LC-MS/MS

Xing Zhang, Daria I. Vernikovskaya, Xiaoming Wang, Tatiana N. Nanovskaya, Maged Costantine, Gary D.V. Hankins, Mahmoud S. Ahmed

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

This report describes the development and validation of a chromatography/tandem mass spectrometry method for the quantitative determination of pravastatin and its metabolite (3α-hydroxy pravastatin) in plasma and urine of pregnant patients under treatment with pravastatin, as part of a clinical trial. The method includes a one-step sample preparation by liquid-liquid extraction. The extraction recovery of the analytes ranged between 93.8 and 99.5% in plasma. The lower limits of quantitation of the analytes in plasma samples were 0.106ng/mL for pravastatin and 0.105ng/mL for 3α-hydroxy pravastatin, while in urine samples they were 19.7ng/mL for pravastatin and 2.00ng/mL for 3α-hydroxy pravastatin. The relative deviation of this method was <10% for intra- and interday assays in plasma and urine samples, and the accuracy ranged between 97.2 and 106% in plasma, and between 98.2 and 105% in urine. The method described in this report was successfully utilized for determining the pharmacokinetics of pravastatin in pregnant patients enrolled in a pilot clinical trial for prevention of preeclampsia.

Original languageEnglish (US)
Pages (from-to)548-554
Number of pages7
JournalBiomedical Chromatography
Volume30
Issue number4
DOIs
StatePublished - Apr 1 2016

Keywords

  • 3α-hydroxy pravastatin
  • LC-MS/MS
  • Metabolite
  • Pravastatin
  • Preeclampsia

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Molecular Biology
  • Pharmacology
  • Drug Discovery
  • Clinical Biochemistry

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