Quantitative determination of six major triterpenoids in Ganoderma lucidum and related species by high performance liquid chromatography

Xiao-Ming Wang, Min Yang, Shu Hong Guan, Rong Xia Liu, Jia Meng Xia, Kai Shun Bi, De An Guo

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

A reversed-phase liquid chromatographic method was developed for the quantitative determination of six triterpenoids, namely ganoderic acids C2, B, AM1, K, H and D in Ganoderma lucidum and its related species. Samples were extracted with chloroform in ultrasonic bath. The optimal conditions of separation and detection were achieved on an Agilent Zorbax SB-C18 column (250 mm × 4.6 mm, 5 μm), with a linear gradient of acetonitrile and 0.03% aqueous phosphoric acid (v/v), at a flow rate of 1.0 ml/min, detected at 252 nm. All calibration curves showed good linearity (r2 > 0.999) within test ranges. The relative deviation of this method was less than 2% for intra- and inter-day assays, and the percentage recovery of the method was 93-103%, with relative standard deviation (R.S.D.) less than 5%. The current assay method was applied to quantitative determination of constituents of triterpenoids in 36 different samples of G. lucidum and its related species. The results indicated that the developed method could be readily utilized as a quality control method for G. lucidum and related species.

Original languageEnglish (US)
Pages (from-to)838-844
Number of pages7
JournalJournal of Pharmaceutical and Biomedical Analysis
Volume41
Issue number3
DOIs
StatePublished - Jun 7 2006
Externally publishedYes

Fingerprint

Reishi
High performance liquid chromatography
Assays
High Pressure Liquid Chromatography
Chloroform
Quality control
Ultrasonics
Flow rate
Calibration
Recovery
Liquids
Baths
Quality Control
ganoderic acid C2
acetonitrile
phosphoric acid

Keywords

  • Ganoderma lucidum
  • Reversed-phase high performance liquid chromatography
  • Triterpenoids

ASJC Scopus subject areas

  • Analytical Chemistry
  • Pharmaceutical Science

Cite this

Quantitative determination of six major triterpenoids in Ganoderma lucidum and related species by high performance liquid chromatography. / Wang, Xiao-Ming; Yang, Min; Guan, Shu Hong; Liu, Rong Xia; Xia, Jia Meng; Bi, Kai Shun; Guo, De An.

In: Journal of Pharmaceutical and Biomedical Analysis, Vol. 41, No. 3, 07.06.2006, p. 838-844.

Research output: Contribution to journalArticle

Wang, Xiao-Ming ; Yang, Min ; Guan, Shu Hong ; Liu, Rong Xia ; Xia, Jia Meng ; Bi, Kai Shun ; Guo, De An. / Quantitative determination of six major triterpenoids in Ganoderma lucidum and related species by high performance liquid chromatography. In: Journal of Pharmaceutical and Biomedical Analysis. 2006 ; Vol. 41, No. 3. pp. 838-844.
@article{4019e1212c72493eab64cd112f84679d,
title = "Quantitative determination of six major triterpenoids in Ganoderma lucidum and related species by high performance liquid chromatography",
abstract = "A reversed-phase liquid chromatographic method was developed for the quantitative determination of six triterpenoids, namely ganoderic acids C2, B, AM1, K, H and D in Ganoderma lucidum and its related species. Samples were extracted with chloroform in ultrasonic bath. The optimal conditions of separation and detection were achieved on an Agilent Zorbax SB-C18 column (250 mm × 4.6 mm, 5 μm), with a linear gradient of acetonitrile and 0.03{\%} aqueous phosphoric acid (v/v), at a flow rate of 1.0 ml/min, detected at 252 nm. All calibration curves showed good linearity (r2 > 0.999) within test ranges. The relative deviation of this method was less than 2{\%} for intra- and inter-day assays, and the percentage recovery of the method was 93-103{\%}, with relative standard deviation (R.S.D.) less than 5{\%}. The current assay method was applied to quantitative determination of constituents of triterpenoids in 36 different samples of G. lucidum and its related species. The results indicated that the developed method could be readily utilized as a quality control method for G. lucidum and related species.",
keywords = "Ganoderma lucidum, Reversed-phase high performance liquid chromatography, Triterpenoids",
author = "Xiao-Ming Wang and Min Yang and Guan, {Shu Hong} and Liu, {Rong Xia} and Xia, {Jia Meng} and Bi, {Kai Shun} and Guo, {De An}",
year = "2006",
month = "6",
day = "7",
doi = "10.1016/j.jpba.2006.01.053",
language = "English (US)",
volume = "41",
pages = "838--844",
journal = "Journal of Pharmaceutical and Biomedical Analysis",
issn = "0731-7085",
publisher = "Elsevier",
number = "3",

}

TY - JOUR

T1 - Quantitative determination of six major triterpenoids in Ganoderma lucidum and related species by high performance liquid chromatography

AU - Wang, Xiao-Ming

AU - Yang, Min

AU - Guan, Shu Hong

AU - Liu, Rong Xia

AU - Xia, Jia Meng

AU - Bi, Kai Shun

AU - Guo, De An

PY - 2006/6/7

Y1 - 2006/6/7

N2 - A reversed-phase liquid chromatographic method was developed for the quantitative determination of six triterpenoids, namely ganoderic acids C2, B, AM1, K, H and D in Ganoderma lucidum and its related species. Samples were extracted with chloroform in ultrasonic bath. The optimal conditions of separation and detection were achieved on an Agilent Zorbax SB-C18 column (250 mm × 4.6 mm, 5 μm), with a linear gradient of acetonitrile and 0.03% aqueous phosphoric acid (v/v), at a flow rate of 1.0 ml/min, detected at 252 nm. All calibration curves showed good linearity (r2 > 0.999) within test ranges. The relative deviation of this method was less than 2% for intra- and inter-day assays, and the percentage recovery of the method was 93-103%, with relative standard deviation (R.S.D.) less than 5%. The current assay method was applied to quantitative determination of constituents of triterpenoids in 36 different samples of G. lucidum and its related species. The results indicated that the developed method could be readily utilized as a quality control method for G. lucidum and related species.

AB - A reversed-phase liquid chromatographic method was developed for the quantitative determination of six triterpenoids, namely ganoderic acids C2, B, AM1, K, H and D in Ganoderma lucidum and its related species. Samples were extracted with chloroform in ultrasonic bath. The optimal conditions of separation and detection were achieved on an Agilent Zorbax SB-C18 column (250 mm × 4.6 mm, 5 μm), with a linear gradient of acetonitrile and 0.03% aqueous phosphoric acid (v/v), at a flow rate of 1.0 ml/min, detected at 252 nm. All calibration curves showed good linearity (r2 > 0.999) within test ranges. The relative deviation of this method was less than 2% for intra- and inter-day assays, and the percentage recovery of the method was 93-103%, with relative standard deviation (R.S.D.) less than 5%. The current assay method was applied to quantitative determination of constituents of triterpenoids in 36 different samples of G. lucidum and its related species. The results indicated that the developed method could be readily utilized as a quality control method for G. lucidum and related species.

KW - Ganoderma lucidum

KW - Reversed-phase high performance liquid chromatography

KW - Triterpenoids

UR - http://www.scopus.com/inward/record.url?scp=33646415921&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33646415921&partnerID=8YFLogxK

U2 - 10.1016/j.jpba.2006.01.053

DO - 10.1016/j.jpba.2006.01.053

M3 - Article

C2 - 16530372

AN - SCOPUS:33646415921

VL - 41

SP - 838

EP - 844

JO - Journal of Pharmaceutical and Biomedical Analysis

JF - Journal of Pharmaceutical and Biomedical Analysis

SN - 0731-7085

IS - 3

ER -